##gff-version 3
#!gff-spec-version 1.21
#!processor NCBI annotwriter
##sequence-region NC_000011.10 1 135086622
##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606
NC_000011.10	RefSeq	region	1	135086622	.	+	.	ID=NC_000011.10:1..135086622;Dbxref=taxon:9606;Name=11;chromosome=11;gbkey=Src;genome=chromosome;mol_type=genomic DNA
NC_000011.10	RefSeqFE	biological_region	5222166	5229620	.	+	.	ID=id-GeneID:107133510;Dbxref=GeneID:107133510;Name=biological region;function=regulatory_interactions: HBB-LCR;gbkey=Region;standard_name=origin of replication at HBB
NC_000011.10	RefSeqFE	origin_of_replication	5222166	5227006	.	.	.	ID=id-GeneID:107133510-2;Dbxref=GeneID:107133510;Note=IR (initiation region)%3B spanning amplicons 59.8 to 65.6%3B identified by PCR analysis or hybridization of BrdU-labelled nascent strands with strand-specific probes;direction=BOTH;experiment=EXISTENCE:nucleotide analog incorporation evidence [ECO:0001107][PMID:7481774];gbkey=rep_origin
NC_000011.10	RefSeqFE	matrix_attachment_site	5222930	5223428	.	.	.	ID=id-GeneID:107133510-3;Dbxref=GeneID:107133510;Note=3'beta SAR%3B inferred minimal region flanked by DdeI and BglII restriction sites;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:2559410%2C PMID:3208739];function=associates with nuclear scaffold;gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	silencer	5223319	5223527	.	.	.	ID=id-GeneID:129662861;Dbxref=GeneID:129662861;Note=fragment chr11:5244549-5244757 (GRCh37/hg19 assembly coordinates);experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:32094911];function=represses an EF-1alpha promoter that drives an FKBP-Casp9 reporter gene in an ReSE screen in HepG2 liver carcinoma cells {active_cell/tissue: HepG2};gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	biological_region	5223319	5223527	.	+	.	ID=id-GeneID:129662861-2;Dbxref=GeneID:129662861;Name=biological region;gbkey=Region;standard_name=ReSE screen-validated silencer GRCh37_chr11:5244549-5244757
NC_000011.10	RefSeqFE	origin_of_replication	5223775	5225457	.	.	.	ID=id-GeneID:107133510-4;Dbxref=GeneID:107133510;Note=bGRep-E%3B EcoRV/SwaI fragment%3B assayed by quantitative PCR of lambda exonuclease-treated size-fractionated nascent strands%3B functions as an origin of replication in an ectopic location;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:16085752];gbkey=rep_origin
NC_000011.10	RefSeqFE	origin_of_replication	5223779	5227023	.	.	.	ID=id-GeneID:107133510-5;Dbxref=GeneID:107133510;Note=bGRep-I%3B NcoI fragment%3B assayed by quantitative PCR of lambda exonuclease-treated size-fractionated nascent strands%3B functions as an origin of replication in ectopic locations;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:15060158];gbkey=rep_origin
NC_000011.10	RefSeqFE	biological_region	5223780	5226590	.	+	.	ID=id-GeneID:110006319;Dbxref=GeneID:110006319;Name=biological region;function=regulatory_interactions: HBB | HBB-LCR;gbkey=Region;standard_name=beta-globin gene 3' regulatory region
NC_000011.10	RefSeqFE	enhancer	5223780	5224891	.	.	.	ID=id-GeneID:110006319-2;Dbxref=GeneID:110006319;Note=1.1 kb HpaII-XbaI fragment deleted in the GB-SH-E transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:2304460];function=enhances expression of a G-gamma-beta hybrid transgene 25-fold in mouse embryonic blood cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5223980	5225041	.	.	.	ID=id-GeneID:110006319-3;Dbxref=GeneID:110006319;Note=1.06 kb BstNI fragment a;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:2828176];function=activates transcription of a G-gamma-globin promoter transgene in mouse fetal liver;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5224441	5224940	.	.	.	ID=id-GeneID:110006319-4;Dbxref=GeneID:110006319;Note=beta-globin 3' enhancer hypersensitive site%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:6200877];gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	enhancer	5224651	5224909	.	.	.	ID=id-GeneID:110006319-5;Dbxref=GeneID:110006319;Note=0.26 kb PstI fragment in relevant transgenes;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8990180%2C PMID:3478680%2C PMID:10807795];function=necessary for expression of the HBB gene in a YAC transgene in fetal liver and adult spleen%3B activates an A-gamma-globin marker transgene in fetal liver and shows an additive effect with an intron 2/exon 3 HBB enhancer element%3B required for LCR activity by 5'HS3 in beta-gamma hybrid transgenes;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	replication_regulatory_region	5224651	5224909	.	.	.	ID=id-GeneID:107133510-6;Dbxref=GeneID:107133510;Note=0.26 kb PstI fragment deleted in the deltaP construct%3B does not have origin activity by itself in a transgene;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:16085752];function=required but not sufficient for replication at the HBB IR origin;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	RefSeqFE	origin_of_replication	5224660	5224899	.	.	.	ID=id-GeneID:107133510-7;Dbxref=GeneID:107133510;Note=amplicon 7%3B peak of nascent strand synthesis detected by quantitative PCR of lambda exonuclease-treated nascent strands;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:16085752];gbkey=rep_origin
NC_000011.10	RefSeqFE	protein_binding_site	5224669	5224694	.	.	.	ID=id-GeneID:110006319-6;Dbxref=GeneID:110006319;Note=oligo D NF-E1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2461328%2C PMID:2911469];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5224704	5224733	.	.	.	ID=id-GeneID:110006319-7;Dbxref=GeneID:110006319;Note=oligo C NF-E1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2461328%2C PMID:2911469];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5224757	5224782	.	.	.	ID=id-GeneID:110006319-8;Dbxref=GeneID:110006319;Note=oligo B NF-E1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2461328%2C PMID:2911469];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5224872	5224897	.	.	.	ID=id-GeneID:110006319-9;Dbxref=GeneID:110006319;Note=oligo A NF-E1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2461328%2C PMID:2911469];gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5224910	5226590	.	.	.	ID=id-GeneID:110006319-10;Dbxref=GeneID:110006319;Note=1.7 kb BamHI-PstI transgene fragment that includes intron 2 and exon 3 of the beta gene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:3478680];function=activates an A-gamma-globin marker transgene in fetal liver and shows an additive effect with the beta 3' enhancer;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5225341	5225840	.	.	.	ID=id-GeneID:110006319-11;Dbxref=GeneID:110006319;Note=hypersensitive site that overlaps the 3' exon of the beta-globin gene%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:6200877];gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	BestRefSeq	gene	5225464	5227071	.	-	.	ID=gene-HBB;Dbxref=GeneID:3043,HGNC:HGNC:4827,MIM:141900;Name=HBB;description=hemoglobin subunit beta;gbkey=Gene;gene=HBB;gene_biotype=protein_coding;gene_synonym=beta-globin,CD113t-C,ECYT6
NC_000011.10	BestRefSeq	mRNA	5225464	5227071	.	-	.	ID=rna-NM_000518.5;Parent=gene-HBB;Dbxref=Ensembl:ENST00000335295.4,GeneID:3043,GenBank:NM_000518.5,HGNC:HGNC:4827,MIM:141900;Name=NM_000518.5;gbkey=mRNA;gene=HBB;product=hemoglobin subunit beta;tag=MANE Select;transcript_id=NM_000518.5
NC_000011.10	BestRefSeq	exon	5226930	5227071	.	-	.	ID=exon-NM_000518.5-1;Parent=rna-NM_000518.5;Dbxref=Ensembl:ENST00000335295.4,GeneID:3043,GenBank:NM_000518.5,HGNC:HGNC:4827,MIM:141900;gbkey=mRNA;gene=HBB;product=hemoglobin subunit beta;tag=MANE Select;transcript_id=NM_000518.5
NC_000011.10	BestRefSeq	exon	5226577	5226799	.	-	.	ID=exon-NM_000518.5-2;Parent=rna-NM_000518.5;Dbxref=Ensembl:ENST00000335295.4,GeneID:3043,GenBank:NM_000518.5,HGNC:HGNC:4827,MIM:141900;gbkey=mRNA;gene=HBB;product=hemoglobin subunit beta;tag=MANE Select;transcript_id=NM_000518.5
NC_000011.10	BestRefSeq	exon	5225464	5225726	.	-	.	ID=exon-NM_000518.5-3;Parent=rna-NM_000518.5;Dbxref=Ensembl:ENST00000335295.4,GeneID:3043,GenBank:NM_000518.5,HGNC:HGNC:4827,MIM:141900;gbkey=mRNA;gene=HBB;product=hemoglobin subunit beta;tag=MANE Select;transcript_id=NM_000518.5
NC_000011.10	BestRefSeq	CDS	5226930	5227021	.	-	0	ID=cds-NP_000509.1;Parent=rna-NM_000518.5;Dbxref=CCDS:CCDS7753.1,Ensembl:ENSP00000333994.3,GeneID:3043,GenBank:NP_000509.1,HGNC:HGNC:4827,MIM:141900;Name=NP_000509.1;gbkey=CDS;gene=HBB;product=hemoglobin subunit beta;protein_id=NP_000509.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5226577	5226799	.	-	1	ID=cds-NP_000509.1;Parent=rna-NM_000518.5;Dbxref=CCDS:CCDS7753.1,Ensembl:ENSP00000333994.3,GeneID:3043,GenBank:NP_000509.1,HGNC:HGNC:4827,MIM:141900;Name=NP_000509.1;gbkey=CDS;gene=HBB;product=hemoglobin subunit beta;protein_id=NP_000509.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5225598	5225726	.	-	0	ID=cds-NP_000509.1;Parent=rna-NM_000518.5;Dbxref=CCDS:CCDS7753.1,Ensembl:ENSP00000333994.3,GeneID:3043,GenBank:NP_000509.1,HGNC:HGNC:4827,MIM:141900;Name=NP_000509.1;gbkey=CDS;gene=HBB;product=hemoglobin subunit beta;protein_id=NP_000509.1;tag=MANE Select
NC_000011.10	RefSeqFE	replication_regulatory_region	5225472	5228126	.	.	.	ID=id-GeneID:107133510-8;Dbxref=GeneID:107133510;Note=core replicator region (position 61100 to position 63754 of J00179);experiment=EXISTENCE:nucleotide analog incorporation evidence [ECO:0001107][PMID:9703500];function=required but not sufficient for replication origin activity in a transgene in an ectopic location;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	RefSeqFE	origin_of_replication	5225673	5229620	.	.	.	ID=id-GeneID:107133510-9;Dbxref=GeneID:107133510;Note=HindIII/EcoRI fragment%3B confers replication activity to a human artificial chromosome (HAC);experiment=EXISTENCE:genetic transformation evidence [ECO:0005027][PMID:9892678];gbkey=rep_origin
NC_000011.10	RefSeqFE	replication_regulatory_region	5225673	5226600	.	.	.	ID=id-GeneID:107133510-10;Dbxref=GeneID:107133510;Note=Rep-I-1 (PmlI/EcoRI fragment);experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:15060158];function=important for replication origin activity;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	RefSeqFE	enhancer	5225674	5226590	.	.	.	ID=id-GeneID:110006319-12;Dbxref=GeneID:110006319;Note=0.9 kb EcoRI-BamHI fragment spanning intron 2%3B region deleted in the H2beta-IVS2 construct;experiment=EXISTENCE:deletion mutation evidence [ECO:0005510][PMID:3432265],EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:10807795];function=required for LCR activity by 5'HS3 along with the beta 3' enhancer in beta-gamma hybrid transgenes%3B increases inducibility of the beta-globin gene in transfected MEL cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5225820	5225959	.	.	.	ID=id-GeneID:110006319-13;Dbxref=GeneID:110006319;Note=active region_4325;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:35858748];function=activates an Ori minimal core promoter by ATAC-STARR-seq in GM12878 lymphoblastoid cells {active_cell/tissue: GM12878};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5225916	5226716	.	.	.	ID=id-GeneID:107133510-11;Dbxref=GeneID:107133510;Note=the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:12397609];gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	matrix_attachment_site	5226074	5226590	.	.	.	ID=id-GeneID:107133510-12;Dbxref=GeneID:107133510;Note=beta-IVS2 SAR;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:2559410%2C PMID:3208739];function=associates with nuclear scaffold;gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	replication_regulatory_region	5226264	5226513	.	.	.	ID=id-GeneID:107133510-13;Dbxref=GeneID:107133510;Note=delta((AG) and(AT)) region%3B includes RepI-1(AG);experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:16877501];function=contributes to replication origin activity;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5226357	5226371	.	.	.	ID=id-GeneID:110006319-14;Dbxref=GeneID:110006319;Note=triplex-forming bases of the beta-globin (L) tfRNA (5'-UUUUUUUUCUUCUCC-3' on minus strand);experiment=EXISTENCE:cell transfection experiment evidence [ECO:0005606][PMID:30867287];function=forms DNA-RNA triplex with 5'HS2 of the HBB-LCR%2C resulting in reduced expression of the espilon- and gamma-globin genes in transfected K562 cells%2C and the gamma- and beta-globin genes in transfected HUDEP-2 cells;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	biological_region	5226570	5228834	.	+	.	ID=id-GeneID:106099062;Dbxref=GeneID:106099062;Name=biological region;function=recombination_partner: LOC106099063 | LOC106099064;gbkey=Region;standard_name=HBB recombination region
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5226570	5226627	.	.	.	ID=id-GeneID:106099062-2;Dbxref=GeneID:106099062;Note=sub-region HBB Lepore-Boston-Washington%2C recombines with sub-region HBD Lepore-Boston-Washington within the HBD recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	origin_of_replication	5226596	5228626	.	.	.	ID=id-GeneID:107133510-14;Dbxref=GeneID:107133510;Note=region containing bidirectional origin of replication between H and I probe fragments%3B flanked by BamHI and BglII restriction sites%3B identified by hybridization of BrdU-labelled nascent strands with strand-specific probes;direction=BOTH;experiment=EXISTENCE:nucleotide analog incorporation evidence [ECO:0001107][PMID:8255298];gbkey=rep_origin
NC_000011.10	RefSeqFE	origin_of_replication	5226611	5227006	.	.	.	ID=id-GeneID:107133510-15;Dbxref=GeneID:107133510;Note=hbetaG amplicon%3B peak of nascent strand synthesis identified by competitive PCR;direction=BOTH;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:10891496],EXISTENCE:nucleotide analog incorporation evidence [ECO:0001107][PMID:9703500];gbkey=rep_origin
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5226631	5226648	.	.	.	ID=id-GeneID:106099062-3;Dbxref=GeneID:106099062;Note=sub-region HBB Lepore-Kenya%2C recombines with sub-region HBD Lepore-Kenya within the HBG1 recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5226638	5226684	.	.	.	ID=id-GeneID:106099062-4;Dbxref=GeneID:106099062;Note=sub-region HBB Lepore-Baltimore%2C recombines with sub-region HBD Lepore-Baltimore within the HBD recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5226642	5226795	.	.	.	ID=id-GeneID:106099062-5;Dbxref=GeneID:106099062;Note=sub-region HBB Lepore-ARUP%2C recombines with sub-region HBD Lepore-ARUP within the HBD recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:23001912];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	meiotic_recombination_region	5226882	5228834	.	.	.	ID=id-GeneID:106099062-6;Dbxref=GeneID:106099062;Note=meiotic double-strand break mapped by DNA meiotic recombinase 1 chromatin immunoprecipitation followed by single-stranded DNA enrichment and sequencing in the germ cells of some male individuals with PRDM9 A/A%2C PRDM9 A/B%2C and PRDM9 A/C genotypes;experiment=EXISTENCE:chromatin immunoprecipitation-seq evidence [ECO:0000229][PMID:25395542];gbkey=misc_recomb;recombination_class=meiotic
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5226915	5226953	.	.	.	ID=id-GeneID:106099062-7;Dbxref=GeneID:106099062;Note=sub-region HBB Lepore-Hollandia%2C recombines with HBD Lepore-Hollandia within the HBD recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	nucleotide_motif	5226958	5226973	.	.	.	ID=id-GeneID:106099062-8;Dbxref=GeneID:106099062;Note=nucleotide motif%3B similarity to the predicted 16-mer PRDM9 C-type binding motif%2C CCNCNNTNNNCNTNNC;feat_class=nucleotide_motif;gbkey=misc_feature
NC_000011.10	RefSeqFE	origin_of_replication	5226986	5227366	.	.	.	ID=id-GeneID:107133510-16;Dbxref=GeneID:107133510;Note=hubetaPr1 amplicon%3B peak of nascent strand synthesis identified by competitive PCR of lambda exonuclease-treated nascent strands;direction=BOTH;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:10891496];gbkey=rep_origin
NC_000011.10	RefSeqFE	origin_of_replication	5227018	5229620	.	.	.	ID=id-GeneID:107133510-17;Dbxref=GeneID:107133510;Note=bGRep-P%3B NcoI fragment%3B assayed by quantitative PCR of lambda exonuclease-treated size-fractionated nascent strands%3B functions as an origin of replication in ectopic locations;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:15060158];gbkey=rep_origin
NC_000011.10	RefSeqFE	replication_regulatory_region	5227018	5227339	.	.	.	ID=id-GeneID:107133510-18;Dbxref=GeneID:107133510;Note=Rep-P-2 (NcoI/SnaBI fragment);experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:15060158];function=important for replication origin activity;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	RefSeqFE	replication_regulatory_region	5227090	5227134	.	.	.	ID=id-GeneID:107133510-19;Dbxref=GeneID:107133510;Note=Rep-P(AG);experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:15060158%2C PMID:16877501];function=contributes to replication origin activity;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	RefSeqFE	replication_regulatory_region	5228528	5229328	.	.	.	ID=id-GeneID:107133510-20;Dbxref=GeneID:107133510;Note=Rep-P-1 (BamHI fragment);experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:15060158];function=important for replication origin activity;gbkey=regulatory;regulatory_class=replication_regulatory_region
NC_000011.10	BestRefSeq	gene	5232838	5234483	.	-	.	ID=gene-HBD;Dbxref=GeneID:3045,HGNC:HGNC:4829,MIM:142000;Name=HBD;description=hemoglobin subunit delta;gbkey=Gene;gene=HBD;gene_biotype=protein_coding;gene_synonym=HBK
NC_000011.10	BestRefSeq	mRNA	5232838	5234483	.	-	.	ID=rna-NM_000519.4;Parent=gene-HBD;Dbxref=Ensembl:ENST00000650601.1,GeneID:3045,GenBank:NM_000519.4,HGNC:HGNC:4829,MIM:142000;Name=NM_000519.4;gbkey=mRNA;gene=HBD;product=hemoglobin subunit delta;tag=MANE Select;transcript_id=NM_000519.4
NC_000011.10	BestRefSeq	exon	5234342	5234483	.	-	.	ID=exon-NM_000519.4-1;Parent=rna-NM_000519.4;Dbxref=Ensembl:ENST00000650601.1,GeneID:3045,GenBank:NM_000519.4,HGNC:HGNC:4829,MIM:142000;gbkey=mRNA;gene=HBD;product=hemoglobin subunit delta;tag=MANE Select;transcript_id=NM_000519.4
NC_000011.10	BestRefSeq	exon	5233991	5234213	.	-	.	ID=exon-NM_000519.4-2;Parent=rna-NM_000519.4;Dbxref=Ensembl:ENST00000650601.1,GeneID:3045,GenBank:NM_000519.4,HGNC:HGNC:4829,MIM:142000;gbkey=mRNA;gene=HBD;product=hemoglobin subunit delta;tag=MANE Select;transcript_id=NM_000519.4
NC_000011.10	BestRefSeq	exon	5232838	5233092	.	-	.	ID=exon-NM_000519.4-3;Parent=rna-NM_000519.4;Dbxref=Ensembl:ENST00000650601.1,GeneID:3045,GenBank:NM_000519.4,HGNC:HGNC:4829,MIM:142000;gbkey=mRNA;gene=HBD;product=hemoglobin subunit delta;tag=MANE Select;transcript_id=NM_000519.4
NC_000011.10	BestRefSeq	CDS	5234342	5234433	.	-	0	ID=cds-NP_000510.1;Parent=rna-NM_000519.4;Dbxref=CCDS:CCDS31376.1,Ensembl:ENSP00000497529.1,GeneID:3045,GenBank:NP_000510.1,HGNC:HGNC:4829,MIM:142000;Name=NP_000510.1;gbkey=CDS;gene=HBD;product=hemoglobin subunit delta;protein_id=NP_000510.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5233991	5234213	.	-	1	ID=cds-NP_000510.1;Parent=rna-NM_000519.4;Dbxref=CCDS:CCDS31376.1,Ensembl:ENSP00000497529.1,GeneID:3045,GenBank:NP_000510.1,HGNC:HGNC:4829,MIM:142000;Name=NP_000510.1;gbkey=CDS;gene=HBD;product=hemoglobin subunit delta;protein_id=NP_000510.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5232964	5233092	.	-	0	ID=cds-NP_000510.1;Parent=rna-NM_000519.4;Dbxref=CCDS:CCDS31376.1,Ensembl:ENSP00000497529.1,GeneID:3045,GenBank:NP_000510.1,HGNC:HGNC:4829,MIM:142000;Name=NP_000510.1;gbkey=CDS;gene=HBD;product=hemoglobin subunit delta;protein_id=NP_000510.1;tag=MANE Select
NC_000011.10	RefSeqFE	biological_region	5233984	5234365	.	+	.	ID=id-GeneID:106099063;Dbxref=GeneID:106099063;Name=biological region;function=recombination_partner: LOC106099062;gbkey=Region;standard_name=HBD recombination region
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5233984	5234041	.	.	.	ID=id-GeneID:106099063-2;Dbxref=GeneID:106099063;Note=sub-region HBD Lepore-Boston-Washington%2C recombines with sub-region HBB Lepore-Boston-Washington within the HBB recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5234052	5234098	.	.	.	ID=id-GeneID:106099063-3;Dbxref=GeneID:106099063;Note=sub-region HBD Lepore-Baltimore%2C recombines with sub-region HBB Lepore-Baltimore within the HBB recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5234154	5234211	.	.	.	ID=id-GeneID:106099063-4;Dbxref=GeneID:106099063;Note=sub-region HBD Lepore-ARUP%2C recombines with sub-region HBB Lepore-ARUP within the HBB recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:23001912];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5234327	5234365	.	.	.	ID=id-GeneID:106099063-5;Dbxref=GeneID:106099063;Note=sub-region HBD Lepore-Hollandia%2C recombines with sub-region HBB Lepore-Hollandia within the HBB recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	biological_region	5234433	5239383	.	+	.	ID=id-GeneID:109951029;Dbxref=GeneID:109951029;Name=biological region;function=regulatory_interactions: HBB | HBD | HBG1 | HBG2;gbkey=Region;standard_name=delta-globin 5' regulatory region
NC_000011.10	RefSeqFE	promoter	5234433	5235258	.	.	.	ID=id-GeneID:109951029-2;Dbxref=GeneID:109951029;Note=826 bp promoter fragment in the p-Ndelta-luc construct;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:22641479];function=promoter for delta-globin (HBD) gene;gbkey=regulatory;regulatory_class=promoter
NC_000011.10	RefSeqFE	promoter	5234473	5234929	.	.	.	ID=id-GeneID:109951029-3;Dbxref=GeneID:109951029;Note=-446 to +11 delta-globin promoter fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:1515647];function=promoter for delta-globin (HBD) gene;gbkey=regulatory;regulatory_class=promoter
NC_000011.10	RefSeqFE	protein_binding_site	5234544	5234573	.	.	.	ID=id-GeneID:109951029-4;Dbxref=GeneID:109951029;Note=DNOR GATA-1-binding oligonucleotide;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:1515647];function=necessary for normal delta-globin promoter activity;gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5234548	5234583	.	.	.	ID=id-GeneID:109951029-5;Dbxref=GeneID:109951029;Note=wild-type delta-globin promoter region with a defective CACCC box and a partial EKLF-binding site%3B promoter activity can be upregulated by restoration of the CCAAT box or by insertion of CACCC motifs;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8916973%2C PMID:9207479];function=reduces activity of the delta-globin (HBD) promoter compared to the equivalent region of the paralogous beta-globin (HBB) gene;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5234556	5234561	.	.	.	ID=id-GeneID:109951029-6;Dbxref=GeneID:109951029;Note=GATA-1 motif that shows impaired delta-globin promoter activity when mutated;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:1515647];function=necessary for GATA-1 binding and normal delta-globin promoter activity;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5235196	5235704	.	.	.	ID=id-GeneID:109951029-7;Dbxref=GeneID:109951029;Note=0.5 kb BsaBI-BsmI fragment removed in the delta transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:9892636];function=facilitates the timing of but is not absolutely required for gamma-to-beta globin gene switching in transgenic mice;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	enhancer	5235323	5235705	.	.	.	ID=id-GeneID:109951029-8;Dbxref=GeneID:109951029;Note=383 bp Sau3AI fragment in the pbeta-cat-delta383 construct%3B includes the entire pyrimidine-rich sequence and the GATA-1 binding site;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8654951];function=enhances beta-globin promoter activity in transfected MEL cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	sequence_secondary_structure	5235380	5235679	.	.	.	ID=id-GeneID:109951029-9;Dbxref=GeneID:109951029;Note=DNA_sequence_secondary_structure%3B likely H-DNA structure that is sensitive to S1 nuclease digestion%3B includes a 250-bp region with >95%25 pyrimidines on the negative strand of the GRCh38 reference genome%3B binds to PYR complex;experiment=EXISTENCE:in vitro cleavage assay evidence [ECO:0001058][PMID:1717993];gbkey=misc_structure
NC_000011.10	RefSeqFE	enhancer	5235389	5235487	.	.	.	ID=id-GeneID:109951029-10;Dbxref=GeneID:109951029;Note=99 bp fragment in the pbeta-cat-delta99 construct%3B includes the PYR binding region but not the GATA-1 binding site;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8654951];function=enhances beta-globin promoter activity in transfected MEL cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5235415	5235474	.	.	.	ID=id-GeneID:109951029-11;Dbxref=GeneID:109951029;Note=delta60 YY1-binding probe;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9892636];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5235667	5235675	.	.	.	ID=id-GeneID:109951029-12;Dbxref=GeneID:109951029;Note=GATA-1 footprint in pyr region;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:1717993];gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5236882	5239383	.	.	.	ID=id-GeneID:109951029-13;Dbxref=GeneID:109951029;Note=2.5 kb BglII fragment deleted in the delta-2.5 kb YAC transgene%3B includes the pyr and R regions;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:10022837%2C PMID:10882078];function=necessary for efficient beta-globin (HBB) gene expression in transgenic mice%3B may also protect the transgene against position effects in fetal liver;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	silencer	5236884	5238501	.	.	.	ID=id-GeneID:109951029-14;Dbxref=GeneID:109951029;Note=1.7 kb R fragment in the betaCAT%2C gammaCAT and gamma-175CAT constructs;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:7524502%2C PMID:11783950];function=negatively regulates the gamma- and beta-globin promoters in transfected MEL cells%2C and the gamma promoter in HEL cells;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	silencer	5236884	5237962	.	.	.	ID=id-GeneID:109951029-15;Dbxref=GeneID:109951029;Note=1 kb F3 fragment in the betaCAT%2C gammaCAT and gamma-175CAT constructs;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:7524502%2C PMID:11783950];function=moderately down-regulates the gamma- and beta-globin promoters in transfected MEL cells%2C and the wild-type gamma promoter in HEL cells;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	silencer	5236884	5237538	.	.	.	ID=id-GeneID:109951029-16;Dbxref=GeneID:109951029;Note=0.65 kb F5 fragment in the betaCAT%2C gammaCAT and gamma-175CAT constructs;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:7524502%2C PMID:11783950];function=strongly down-regulates the beta-globin promoter in transfected MEL cells%2C and the wild-type gamma-globin promoter in HEL cells;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	protein_binding_site	5237105	5237142	.	.	.	ID=id-GeneID:109951029-17;Dbxref=GeneID:109951029;Note=oligo 38 ALUdelta-2 GATA-1-binding probe;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:11783950];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5237387	5237438	.	.	.	ID=id-GeneID:109951029-18;Dbxref=GeneID:109951029;Note=51-bp GATA-1-binding oligo;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:11783950];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5238130	5238167	.	.	.	ID=id-GeneID:109951029-19;Dbxref=GeneID:109951029;Note=oligo 38 ALUdelta-1 GATA-1-binding probe;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:11783950];gbkey=protein_bind
NC_000011.10	BestRefSeq	pseudogene	5241954	5243592	.	-	.	ID=gene-HBBP1;Dbxref=GeneID:3044,HGNC:HGNC:4828;Name=HBBP1;description=hemoglobin subunit beta pseudogene 1;gbkey=Gene;gene=HBBP1;gene_biotype=transcribed_pseudogene;gene_synonym=HBH1,HBHP,HBHps;pseudo=true
NC_000011.10	BestRefSeq	transcript	5241954	5243592	.	-	.	ID=rna-NR_001589.1;Parent=gene-HBBP1;Dbxref=GeneID:3044,GenBank:NR_001589.1,HGNC:HGNC:4828;Name=NR_001589.1;gbkey=misc_RNA;gene=HBBP1;product=hemoglobin subunit beta pseudogene 1;pseudo=true;transcript_id=NR_001589.1
NC_000011.10	BestRefSeq	exon	5243450	5243592	.	-	.	ID=exon-NR_001589.1-1;Parent=rna-NR_001589.1;Dbxref=GeneID:3044,GenBank:NR_001589.1,HGNC:HGNC:4828;gbkey=misc_RNA;gene=HBBP1;product=hemoglobin subunit beta pseudogene 1;pseudo=true;transcript_id=NR_001589.1
NC_000011.10	BestRefSeq	exon	5243106	5243328	.	-	.	ID=exon-NR_001589.1-2;Parent=rna-NR_001589.1;Dbxref=GeneID:3044,GenBank:NR_001589.1,HGNC:HGNC:4828;gbkey=misc_RNA;gene=HBBP1;product=hemoglobin subunit beta pseudogene 1;pseudo=true;transcript_id=NR_001589.1
NC_000011.10	BestRefSeq	exon	5241954	5242247	.	-	.	ID=exon-NR_001589.1-3;Parent=rna-NR_001589.1;Dbxref=GeneID:3044,GenBank:NR_001589.1,HGNC:HGNC:4828;gbkey=misc_RNA;gene=HBBP1;product=hemoglobin subunit beta pseudogene 1;pseudo=true;transcript_id=NR_001589.1
NC_000011.10	BestRefSeq	gene	5244554	5245546	.	-	.	ID=gene-BGLT3;Dbxref=GeneID:103344929,HGNC:HGNC:49033,MIM:616308;Name=BGLT3;description=beta globin locus transcript 3;gbkey=Gene;gene=BGLT3;gene_biotype=lncRNA;gene_synonym=BGL3,LINC01083,lncRNA-BGL3
NC_000011.10	BestRefSeq	lncRNA	5244554	5245546	.	-	.	ID=rna-NR_121648.1;Parent=gene-BGLT3;Dbxref=GeneID:103344929,GenBank:NR_121648.1,HGNC:HGNC:49033,MIM:616308;Name=NR_121648.1;gbkey=ncRNA;gene=BGLT3;product=beta globin locus transcript 3;transcript_id=NR_121648.1
NC_000011.10	BestRefSeq	exon	5244554	5245546	.	-	.	ID=exon-NR_121648.1-1;Parent=rna-NR_121648.1;Dbxref=GeneID:103344929,GenBank:NR_121648.1,HGNC:HGNC:49033,MIM:616308;gbkey=ncRNA;gene=BGLT3;product=beta globin locus transcript 3;transcript_id=NR_121648.1
NC_000011.10	RefSeqFE	biological_region	5245527	5248345	.	+	.	ID=id-GeneID:109951028;Dbxref=GeneID:109951028;Name=biological region;function=regulatory_interactions: HBE1 | HBG1 | HBG2;gbkey=Region;standard_name=A-gamma-globin 3' regulatory region
NC_000011.10	RefSeqFE	enhancer	5245527	5247879	.	.	.	ID=id-GeneID:109951028-2;Dbxref=GeneID:109951028;Note=2.3 kb EcoRI fragment 11;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:3691478];function=enhances G-gamma-globin promoter activity in transfected K562%2C HEL%2C HeLa and 293 cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	silencer	5246294	5247897	.	.	.	ID=id-GeneID:109951028-3;Dbxref=GeneID:109951028;Note=1.6 kb fragment containing the Enh and F elements that are deleted in the delta-Enh/F transgene%3B note that this region does not appear to be necessary when deleted in the context of a YAC transgene (PMIDs 9707580 and 12629213);experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:15102485%2C PMID:19690621];function=necessary for down-regulating the epsilon- and gamma-globin genes in embryonic yolk sac of transgenic mice%2C but not for fetal to adult beta-globin gene switching%3B also silences fetal gamma-globin expression in adult transgenic mice;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	silencer	5246294	5246663	.	.	.	ID=id-GeneID:109951028-4;Dbxref=GeneID:109951028;Note=F element;experiment=COORDINATES:manual assertion [ECO:0000218][PMID:15102485],EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8422748];function=negatively regulates the Rous Sarcoma Virus LTR promoter in transfected K562 erythroleukemia cells;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5246395	5248345	.	.	.	ID=id-GeneID:109951028-5;Dbxref=GeneID:109951028;Note=A-gamma 3' DNase I hypersensitive region that includes three sub-bands (B%2C C and D)%3B observed in erythroleukemia K562 cells but not in promyelocytic HL60 or monocytoid U937 cells%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:3691478];gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	enhancer	5247136	5247879	.	.	.	ID=id-GeneID:109951028-6;Dbxref=GeneID:109951028;Note=0.75 kb EcoRI/HindIII enhancer fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:3691478];function=enhances G-gamma-globin promoter activity in transfected K562 cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	silencer	5247144	5247897	.	.	.	ID=id-GeneID:109951028-7;Dbxref=GeneID:109951028;Note=Enh element;experiment=COORDINATES:manual assertion [ECO:0000218][PMID:15102485],EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8422748];function=negatively regulates the Rous Sarcoma Virus LTR promoter in transfected K562 erythroleukemia cells;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	insulator	5247311	5247888	.	.	.	ID=id-GeneID:109951028-8;Dbxref=GeneID:109951028;Note=0.6 kb NciI-HindIII minimal fragment necessary for protecting transgenes with a micro-LCR against position effects;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8972204];function=shelters gamma-globin expression from position effects in transgenes with a micro-LCR;gbkey=regulatory;regulatory_class=insulator
NC_000011.10	RefSeqFE	protein_binding_site	5247335	5247360	.	.	.	ID=id-GeneID:109951028-9;Dbxref=GeneID:109951028;Note=footprint VII GATA-1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2259631];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5247419	5247445	.	.	.	ID=id-GeneID:109951028-10;Dbxref=GeneID:109951028;Note=footprint V GATA-1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2259631];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5247489	5247585	.	.	.	ID=id-GeneID:109951028-11;Dbxref=GeneID:109951028;Note=RsaI-AvaII 3'gammaPE2 fragment that binds both SATB1 and HOXB2;bound_moiety=homeobox B2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:10595394];function=negatively affects transcription of a transiently transfected gamma-globin reporter construct;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5247549	5247585	.	.	.	ID=id-GeneID:109951028-12;Dbxref=GeneID:109951028;Note=SATB1 site IV fragment%3B overlaps the 3'gammaPE2 fragment (PMID:10595394);bound_moiety=SATB homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8049444%2C PMID:10595394];function=positively affects transcription of a transiently transfected gamma-globin reporter construct;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5247738	5247766	.	.	.	ID=id-GeneID:109951028-13;Dbxref=GeneID:109951028;Note=footprint II GATA-1-binding fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2259631];gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5247815	5247856	.	.	.	ID=id-GeneID:109951028-14;Dbxref=GeneID:109951028;Note=3'gammaPE1-42 fragment in the -161 gammaLUC-42-10 construct;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8034705];function=increases transcriptional activity of a gamma-globin or SV40 heterologus promoter in transfected HeLa or K562 cells;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5247815	5247856	.	.	.	ID=id-GeneID:109951028-15;Dbxref=GeneID:109951028;Note=3'gammaPE1 fragment that binds both SATB1 and HOXB2;bound_moiety=homeobox B2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:10595394];function=negatively affects transcription of a transiently transfected gamma-globin reporter construct;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5247815	5247856	.	.	.	ID=id-GeneID:109951028-16;Dbxref=GeneID:109951028;Note=3'gammaPE1 fragment that binds both SATB1 and HOXB2%3B overlaps the SATB1 site I fragment (PMID:8049444);bound_moiety=SATB homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:10595394%2C PMID:8049444];function=positively affects transcription of a transiently transfected gamma-globin reporter construct;gbkey=protein_bind
NC_000011.10	BestRefSeq	gene	5248269	5249857	.	-	.	ID=gene-HBG1;Dbxref=GeneID:3047,HGNC:HGNC:4831,MIM:142200;Name=HBG1;description=hemoglobin subunit gamma 1;gbkey=Gene;gene=HBG1;gene_biotype=protein_coding;gene_synonym=HBG-T2,HBGA,HBGR,HSGGL1,PRO2979
NC_000011.10	BestRefSeq	mRNA	5248269	5249857	.	-	.	ID=rna-NM_000559.3;Parent=gene-HBG1;Dbxref=Ensembl:ENST00000330597.5,GeneID:3047,GenBank:NM_000559.3,HGNC:HGNC:4831,MIM:142200;Name=NM_000559.3;gbkey=mRNA;gene=HBG1;product=hemoglobin subunit gamma 1;tag=MANE Select;transcript_id=NM_000559.3
NC_000011.10	BestRefSeq	exon	5249713	5249857	.	-	.	ID=exon-NM_000559.3-1;Parent=rna-NM_000559.3;Dbxref=Ensembl:ENST00000330597.5,GeneID:3047,GenBank:NM_000559.3,HGNC:HGNC:4831,MIM:142200;gbkey=mRNA;gene=HBG1;product=hemoglobin subunit gamma 1;tag=MANE Select;transcript_id=NM_000559.3
NC_000011.10	BestRefSeq	exon	5249368	5249590	.	-	.	ID=exon-NM_000559.3-2;Parent=rna-NM_000559.3;Dbxref=Ensembl:ENST00000330597.5,GeneID:3047,GenBank:NM_000559.3,HGNC:HGNC:4831,MIM:142200;gbkey=mRNA;gene=HBG1;product=hemoglobin subunit gamma 1;tag=MANE Select;transcript_id=NM_000559.3
NC_000011.10	BestRefSeq	exon	5248269	5248487	.	-	.	ID=exon-NM_000559.3-3;Parent=rna-NM_000559.3;Dbxref=Ensembl:ENST00000330597.5,GeneID:3047,GenBank:NM_000559.3,HGNC:HGNC:4831,MIM:142200;gbkey=mRNA;gene=HBG1;product=hemoglobin subunit gamma 1;tag=MANE Select;transcript_id=NM_000559.3
NC_000011.10	BestRefSeq	CDS	5249713	5249804	.	-	0	ID=cds-NP_000550.2;Parent=rna-NM_000559.3;Dbxref=CCDS:CCDS7754.1,Ensembl:ENSP00000327431.4,GeneID:3047,GenBank:NP_000550.2,HGNC:HGNC:4831,MIM:142200;Name=NP_000550.2;gbkey=CDS;gene=HBG1;product=hemoglobin subunit gamma-1;protein_id=NP_000550.2;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5249368	5249590	.	-	1	ID=cds-NP_000550.2;Parent=rna-NM_000559.3;Dbxref=CCDS:CCDS7754.1,Ensembl:ENSP00000327431.4,GeneID:3047,GenBank:NP_000550.2,HGNC:HGNC:4831,MIM:142200;Name=NP_000550.2;gbkey=CDS;gene=HBG1;product=hemoglobin subunit gamma-1;protein_id=NP_000550.2;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5248359	5248487	.	-	0	ID=cds-NP_000550.2;Parent=rna-NM_000559.3;Dbxref=CCDS:CCDS7754.1,Ensembl:ENSP00000327431.4,GeneID:3047,GenBank:NP_000550.2,HGNC:HGNC:4831,MIM:142200;Name=NP_000550.2;gbkey=CDS;gene=HBG1;product=hemoglobin subunit gamma-1;protein_id=NP_000550.2;tag=MANE Select
NC_000011.10	RefSeqFE	biological_region	5249068	5250324	.	+	.	ID=id-GeneID:106099064;Dbxref=GeneID:106099064;Name=biological region;function=recombination_partner: LOC106099062 | LOC106099065;gbkey=Region;standard_name=HBG1 recombination region
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5249068	5250079	.	.	.	ID=id-GeneID:106099064-2;Dbxref=GeneID:106099064;Note=sub-region a%2C recombines with sub-region a' within the HBG2 recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5249422	5249439	.	.	.	ID=id-GeneID:106099064-3;Dbxref=GeneID:106099064;Note=sub-region HBG1 Lepore-Kenya%2C recombines with sub-region HBB Lepore-Kenya within the HBB recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5250252	5250324	.	.	.	ID=id-GeneID:106099064-4;Dbxref=GeneID:106099064;Note=sub-region b%2C recombines with sub-region b' within the HBG2 recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:3401591];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	BestRefSeq	gene	5253188	5254781	.	-	.	ID=gene-HBG2;Dbxref=GeneID:3048,HGNC:HGNC:4832,MIM:142250;Name=HBG2;description=hemoglobin subunit gamma 2;gbkey=Gene;gene=HBG2;gene_biotype=protein_coding;gene_synonym=HBG-T1,TNCY
NC_000011.10	BestRefSeq	mRNA	5253188	5254781	.	-	.	ID=rna-NM_000184.3;Parent=gene-HBG2;Dbxref=Ensembl:ENST00000336906.6,GeneID:3048,GenBank:NM_000184.3,HGNC:HGNC:4832,MIM:142250;Name=NM_000184.3;gbkey=mRNA;gene=HBG2;product=hemoglobin subunit gamma 2;tag=MANE Select;transcript_id=NM_000184.3
NC_000011.10	BestRefSeq	exon	5254637	5254781	.	-	.	ID=exon-NM_000184.3-1;Parent=rna-NM_000184.3;Dbxref=Ensembl:ENST00000336906.6,GeneID:3048,GenBank:NM_000184.3,HGNC:HGNC:4832,MIM:142250;gbkey=mRNA;gene=HBG2;product=hemoglobin subunit gamma 2;tag=MANE Select;transcript_id=NM_000184.3
NC_000011.10	BestRefSeq	exon	5254292	5254514	.	-	.	ID=exon-NM_000184.3-2;Parent=rna-NM_000184.3;Dbxref=Ensembl:ENST00000336906.6,GeneID:3048,GenBank:NM_000184.3,HGNC:HGNC:4832,MIM:142250;gbkey=mRNA;gene=HBG2;product=hemoglobin subunit gamma 2;tag=MANE Select;transcript_id=NM_000184.3
NC_000011.10	BestRefSeq	exon	5253188	5253405	.	-	.	ID=exon-NM_000184.3-3;Parent=rna-NM_000184.3;Dbxref=Ensembl:ENST00000336906.6,GeneID:3048,GenBank:NM_000184.3,HGNC:HGNC:4832,MIM:142250;gbkey=mRNA;gene=HBG2;product=hemoglobin subunit gamma 2;tag=MANE Select;transcript_id=NM_000184.3
NC_000011.10	BestRefSeq	CDS	5254637	5254728	.	-	0	ID=cds-NP_000175.1;Parent=rna-NM_000184.3;Dbxref=CCDS:CCDS7755.1,Ensembl:ENSP00000338082.4,GeneID:3048,GenBank:NP_000175.1,HGNC:HGNC:4832,MIM:142250;Name=NP_000175.1;gbkey=CDS;gene=HBG2;product=hemoglobin subunit gamma-2;protein_id=NP_000175.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5254292	5254514	.	-	1	ID=cds-NP_000175.1;Parent=rna-NM_000184.3;Dbxref=CCDS:CCDS7755.1,Ensembl:ENSP00000338082.4,GeneID:3048,GenBank:NP_000175.1,HGNC:HGNC:4832,MIM:142250;Name=NP_000175.1;gbkey=CDS;gene=HBG2;product=hemoglobin subunit gamma-2;protein_id=NP_000175.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5253277	5253405	.	-	0	ID=cds-NP_000175.1;Parent=rna-NM_000184.3;Dbxref=CCDS:CCDS7755.1,Ensembl:ENSP00000338082.4,GeneID:3048,GenBank:NP_000175.1,HGNC:HGNC:4832,MIM:142250;Name=NP_000175.1;gbkey=CDS;gene=HBG2;product=hemoglobin subunit gamma-2;protein_id=NP_000175.1;tag=MANE Select
NC_000011.10	RefSeqFE	biological_region	5253987	5255252	.	+	.	ID=id-GeneID:106099065;Dbxref=GeneID:106099065;Name=biological region;function=recombination_partner: LOC106099064;gbkey=Region;standard_name=HBG2 recombination region
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5253987	5255003	.	.	.	ID=id-GeneID:106099065-2;Dbxref=GeneID:106099065;Note=sub-region a'%2C recombines with sub-region a within the HBG1 recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:2060774];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	non_allelic_homologous_recombination_region	5255180	5255252	.	.	.	ID=id-GeneID:106099065-3;Dbxref=GeneID:106099065;Note=sub-region b'%2C recombines with sub-region b within the HBG1 recombination region;experiment=EXISTENCE:nucleotide sequencing assay evidence [ECO:0000219][PMID:3401591];gbkey=misc_recomb;recombination_class=non_allelic_homologous
NC_000011.10	RefSeqFE	enhancer	5257261	5257876	.	.	.	ID=id-GeneID:127820280;Dbxref=GeneID:127820280;Note=OCT4-NANOG hESC enhancer chr11:5278491-5279106 (GRCh37/hg19 assembly coordinates);experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:30033119];function=activates a minimal SCP1%2C AML or CMV promoter by ChIP-STARR-seq in primed H9 embryonic stem cells {active_cell/tissue: H9 hESC(primed)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	biological_region	5257261	5257876	.	+	.	ID=id-GeneID:127820280-2;Dbxref=GeneID:127820280;Name=biological region;gbkey=Region;standard_name=OCT4-NANOG hESC enhancer GRCh37_chr11:5278491-5279106
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5266773	5267519	.	.	.	ID=id-GeneID:110740339;Dbxref=GeneID:110740339;Note=hypersensitive site HS-E1%3B observed in K562 erythroleukemia cells%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:21321362];gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	biological_region	5266773	5267519	.	+	.	ID=id-GeneID:110740339-2;Dbxref=GeneID:110740339;Name=biological region;gbkey=Region;standard_name=beta-globin hypersensitive site-embryonic 1
NC_000011.10	RefSeqFE	conserved_region	5266773	5267519	.	.	.	ID=id-GeneID:110740339-3;Dbxref=GeneID:110740339;Note=conserved_region%3B human and mouse orthologous HS-E1 region;experiment=EXISTENCE:sequence alignment evidence [ECO:0000200][PMID:21321362];feat_class=conserved_region;gbkey=misc_feature
NC_000011.10	BestRefSeq	gene	5268345	5269945	.	-	.	ID=gene-HBE1;Dbxref=GeneID:3046,HGNC:HGNC:4830,MIM:142100;Name=HBE1;description=hemoglobin subunit epsilon 1;gbkey=Gene;gene=HBE1;gene_biotype=protein_coding;gene_synonym=HBE
NC_000011.10	BestRefSeq	mRNA	5268345	5269945	.	-	.	ID=rna-NM_005330.4;Parent=gene-HBE1;Dbxref=Ensembl:ENST00000396895.3,GeneID:3046,GenBank:NM_005330.4,HGNC:HGNC:4830,MIM:142100;Name=NM_005330.4;gbkey=mRNA;gene=HBE1;product=hemoglobin subunit epsilon 1;tag=MANE Select;transcript_id=NM_005330.4
NC_000011.10	BestRefSeq	exon	5269799	5269945	.	-	.	ID=exon-NM_005330.4-1;Parent=rna-NM_005330.4;Dbxref=Ensembl:ENST00000396895.3,GeneID:3046,GenBank:NM_005330.4,HGNC:HGNC:4830,MIM:142100;gbkey=mRNA;gene=HBE1;product=hemoglobin subunit epsilon 1;tag=MANE Select;transcript_id=NM_005330.4
NC_000011.10	BestRefSeq	exon	5269454	5269676	.	-	.	ID=exon-NM_005330.4-2;Parent=rna-NM_005330.4;Dbxref=Ensembl:ENST00000396895.3,GeneID:3046,GenBank:NM_005330.4,HGNC:HGNC:4830,MIM:142100;gbkey=mRNA;gene=HBE1;product=hemoglobin subunit epsilon 1;tag=MANE Select;transcript_id=NM_005330.4
NC_000011.10	BestRefSeq	exon	5268345	5268597	.	-	.	ID=exon-NM_005330.4-3;Parent=rna-NM_005330.4;Dbxref=Ensembl:ENST00000396895.3,GeneID:3046,GenBank:NM_005330.4,HGNC:HGNC:4830,MIM:142100;gbkey=mRNA;gene=HBE1;product=hemoglobin subunit epsilon 1;tag=MANE Select;transcript_id=NM_005330.4
NC_000011.10	BestRefSeq	CDS	5269799	5269890	.	-	0	ID=cds-NP_005321.1;Parent=rna-NM_005330.4;Dbxref=CCDS:CCDS7756.1,Ensembl:ENSP00000380104.2,GeneID:3046,GenBank:NP_005321.1,HGNC:HGNC:4830,MIM:142100;Name=NP_005321.1;gbkey=CDS;gene=HBE1;product=hemoglobin subunit epsilon;protein_id=NP_005321.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5269454	5269676	.	-	1	ID=cds-NP_005321.1;Parent=rna-NM_005330.4;Dbxref=CCDS:CCDS7756.1,Ensembl:ENSP00000380104.2,GeneID:3046,GenBank:NP_005321.1,HGNC:HGNC:4830,MIM:142100;Name=NP_005321.1;gbkey=CDS;gene=HBE1;product=hemoglobin subunit epsilon;protein_id=NP_005321.1;tag=MANE Select
NC_000011.10	BestRefSeq	CDS	5268469	5268597	.	-	0	ID=cds-NP_005321.1;Parent=rna-NM_005330.4;Dbxref=CCDS:CCDS7756.1,Ensembl:ENSP00000380104.2,GeneID:3046,GenBank:NP_005321.1,HGNC:HGNC:4830,MIM:142100;Name=NP_005321.1;gbkey=CDS;gene=HBE1;product=hemoglobin subunit epsilon;protein_id=NP_005321.1;tag=MANE Select
NC_000011.10	RefSeqFE	biological_region	5269925	5304186	.	+	.	ID=id-GeneID:109580095;Dbxref=GeneID:109580095;Name=biological region;function=regulatory_interactions: HBB | HBBP1 | HBD | HBE1 | HBG1 | HBG2 | LOC107133510 | LOC110006319;gbkey=Region;standard_name=beta-globin locus control region
NC_000011.10	RefSeqFE	promoter	5269925	5271393	.	.	.	ID=id-GeneID:109580095-2;Dbxref=GeneID:109580095;Note=+19 to -1450 p-epsilon-GLCAT construct fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:3456225%2C PMID:4048224];function=epsilon gene (HBE1) full promoter;gbkey=regulatory;regulatory_class=promoter
NC_000011.10	RefSeqFE	promoter	5269925	5270121	.	.	.	ID=id-GeneID:109580095-3;Dbxref=GeneID:109580095;Note=+19 to -177 p-epsilon-delta177 construct fragment lacking the silencer region;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:2748586];function=epsilon gene (HBE1) minimal promoter;gbkey=regulatory;regulatory_class=promoter
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5269943	5270410	.	.	.	ID=id-GeneID:109580095-4;Dbxref=GeneID:109580095;Note=range from the chr11.787.32 to chr11.787.2.2 gRNAs in the HBE1 promoter region targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28369033];function=positively regulates HBE1 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	TATA_box	5269971	5269977	.	.	.	ID=id-GeneID:109580095-5;Dbxref=GeneID:109580095;Note=based on paralogous and orthologous beta-globin promoter alignments;experiment=EXISTENCE:sequence alignment evidence [ECO:0000200][PMID:6985477];function=epsilon gene (HBE1) TATA box;gbkey=regulatory;regulatory_class=TATA_box
NC_000011.10	RefSeqFE	CAAT_signal	5270024	5270028	.	.	.	ID=id-GeneID:109580095-6;Dbxref=GeneID:109580095;Note=based on paralogous and orthologous beta-globin promoter alignments;experiment=EXISTENCE:sequence alignment evidence [ECO:0000200][PMID:6985477];function=epsilon gene (HBE1) CCAAT box;gbkey=regulatory;regulatory_class=CAAT_signal
NC_000011.10	RefSeqFE	silencer	5270121	5270402	.	.	.	ID=id-GeneID:109580095-7;Dbxref=GeneID:109580095;Note=-182 to -467 AflII-BamHI fragment;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:1737096];function=epsilon gene (HBE1) silencer;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	silencer	5270122	5270336	.	.	.	ID=id-GeneID:109580095-8;Dbxref=GeneID:109580095;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:2748586];function=epsilon gene (HBE1) silencer;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	silencer	5270195	5270238	.	.	.	ID=id-GeneID:109580095-9;Dbxref=GeneID:109580095;Note=-294 to -251 44 bp minimal silencer element;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:1537556];function=epsilon gene (HBE1) minimal silencer;gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	protein_binding_site	5270258	5270282	.	.	.	ID=id-GeneID:109580095-10;Dbxref=GeneID:109580095;Note=M-epsilon MAR SATB1 binding site;bound_moiety=SATB homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:15618465];function=enhances transcriptional activity of the epsilon promoter;gbkey=protein_bind
NC_000011.10	RefSeqFE	locus_control_region	5270791	5292315	.	.	.	ID=id-GeneID:109580095-11;Dbxref=GeneID:109580095;Note=21.5 kb ClaI-BglII fragment from -1 kb to -22.5 kb%3B includes 5'HS1-5'HS5;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:3690667];function=regulates developmental expression of the beta-globin genes;gbkey=regulatory;regulatory_class=locus_control_region
NC_000011.10	RefSeqFE	matrix_attachment_site	5273038	5274981	.	.	.	ID=id-GeneID:109580095-12;Dbxref=GeneID:109580095;Note=2 kb AvaII pGSE228 fragment c SAR;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:3208739%2C PMID:2559410];function=associates with the nuclear scaffold;gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	enhancer	5275198	5276133	.	.	.	ID=id-GeneID:109580095-13;Dbxref=GeneID:109580095;Note=5'HS1 or HS1-3' enhancer fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:20231293%2C PMID:17548470];function=enhancer in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5275669	5276496	.	.	.	ID=id-GeneID:109580095-14;Dbxref=GeneID:109580095;Note=0.8 kb NcoI-HindIII fragment deleted in the delta-5'HS1 beta-YAC transgene;experiment=EXISTENCE:deletion mutation evidence [ECO:0005510][PMID:17433733];function=enhances epsilon- and gamma-globin gene expression in embryonic and fetal stage transgenic mice;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5275745	5276302	.	.	.	ID=id-GeneID:109580095-15;Dbxref=GeneID:109580095;Note=5'HS1%2C also known as HS1%2C HSS1%2C HSI or -6.1 hypersensitive site%3B predominantly erythroid%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:2370867%2C PMID:3879975%2C PMID:3480506%2C PMID:3690667];function=necesssary for position-independent and copy number-dependent activity of the LCR in transgenic mice;gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5275754	5276075	.	.	.	ID=id-GeneID:109580095-16;Dbxref=GeneID:109580095;Note=TAD1.SE1.HS1 sgRNA1-sgRNA3 range targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28416141];function=positively regulates HBG2 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5275811	5276137	.	.	.	ID=id-GeneID:109580095-17;Dbxref=GeneID:109580095;Note=range from the chr11.788.9 to chr11.788.4 gRNAs in the HS1 region targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28369033];function=positively regulates HBE1 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	enhancer	5275915	5276048	.	.	.	ID=id-GeneID:109580095-18;Dbxref=GeneID:109580095;Note=5'HS1 G fragment with strongest enhancer activity;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:20231293];function=enhancer in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5275917	5275929	.	.	.	ID=id-GeneID:109580095-19;Dbxref=GeneID:109580095;Note=probe G GATA-1 binding site;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9657768];gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5275934	5276023	.	.	.	ID=id-GeneID:109580095-20;Dbxref=GeneID:109580095;Note=5'HS1 N fragment with enhancer activity%3B corresponds to the combined K and L fragments;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:20231293];function=enhancer in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5275952	5275970	.	.	.	ID=id-GeneID:109580095-21;Dbxref=GeneID:109580095;Note=L2 fragment or probe F GATA-1 binding site;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:20231293%2C PMID:9657768];function=necessary for efficient epsilon-globin (HBE1) gene expression;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5275984	5276006	.	.	.	ID=id-GeneID:109580095-22;Dbxref=GeneID:109580095;Note=probe E Sp1/Sp3 binding site;bound_moiety=Sp1 or Sp3 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9657768];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5276138	5276156	.	.	.	ID=id-GeneID:109580095-23;Dbxref=GeneID:109580095;Note=probe C GATA-1 binding site;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9657768];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5276225	5276253	.	.	.	ID=id-GeneID:109580095-24;Dbxref=GeneID:109580095;Note=probe A GATA-1 binding site;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9657768];gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5279020	5280939	.	.	.	ID=id-GeneID:109580095-25;Dbxref=GeneID:109580095;Note=1.9 kb HindIII HS2 enhancer fragment in the HS2-Ggamma-Agamma-delta-beta construct;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:9002975%2C PMID:8692864];function=intermediate rate globin gene switching in transgenic mice;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5279781	5281666	.	.	.	ID=id-GeneID:109580095-26;Dbxref=GeneID:109580095;Note=1.9 kb KpnI-PvuII HS2 enhancer fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:10196210],EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:9002975];function=acts in concert with the HS3 enhancer to allow stage-specific expression of the gamma- and beta-globin genes in transfected HEL and K562 cells%3B functions in globin gene switching in transgenic mice {active_cell/tissue: HEL | K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	matrix_attachment_site	5279915	5280427	.	.	.	ID=id-GeneID:109580095-27;Dbxref=GeneID:109580095;Note=514 bp HS2 amplicon showing matrix enrichment in K562 cell EcoRI/HindIII-digested DNA fractions;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:12799453];gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	enhancer	5280212	5280939	.	.	.	ID=id-GeneID:109580095-28;Dbxref=GeneID:109580095;Note=BglII-HindIII HS2 enhancer fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:15979088];function=enhances transcriptional activity of beta-globin genes;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	sequence_secondary_structure	5280348	5280527	.	.	.	ID=id-GeneID:109580095-29;Dbxref=GeneID:109580095;Note=intrinsic bending region%3B epsilonB-16 DNA bend center flanking HS2;experiment=EXISTENCE:gel electrophoresis evidence [ECO:0000337][PMID:9837721];function=nucleosome phasing at HS2;gbkey=misc_structure
NC_000011.10	RefSeqFE	enhancer	5280484	5286050	.	.	.	ID=id-GeneID:109580095-30;Dbxref=GeneID:109580095;Note=MfeI-HpaI HS2-HS3 enhancer fragment in the HS23ALL construct;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:14636653];function=regulates beta-globin gene spatial and temporal expression in transgenic mice%3B synergistic regulation by HS2 and HS3 combined;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5280484	5283752	.	.	.	ID=id-GeneID:109580095-31;Dbxref=GeneID:109580095;Note=MfeI-MfeI HS2 fragment in the HS2ALL construct;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:14636653];function=enhances beta-globin gene expression in embyonic yolk sac%2C fetal liver and adult bone marrow of transgenic mice {active_cell/tissue: yolk sac(embryonic) | liver(fetal) | bone marrow(adult)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5280548	5281017	.	.	.	ID=id-GeneID:109580095-32;Dbxref=GeneID:109580095;Note=5'HS2%2C also known as HS2%2C HSS2%2C HSII%2C -10.8 or -10.9 hypersensitive site%3B not exclusively erythroid%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:2370867%2C PMID:3879975%2C PMID:3480506%2C PMID:3690667];function=necessary for high-level beta-globin gene expression and for position-independent and copy number-dependent activity of the LCR in transgenic mice;gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	enhancer	5280570	5280944	.	.	.	ID=id-GeneID:109580095-33;Dbxref=GeneID:109580095;Note=0.4 kb HindIII-XbaI HS2 fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8662652%2C PMID:11158302%2C PMID:12582237];function=enhances transcription of the epsilon- and beta-globin genes in transfected K562 cells%3B EKLF-dependent activity {active_cell/tissue: K562(+EKLF)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280570	5280919	.	.	.	ID=id-GeneID:109580095-34;Dbxref=GeneID:109580095;Note=range of 21 sgRNAs (Cr1-Cr21) in the HS2 region targeted for CRISPR/dCas9-KRAB perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:26501517];function=positively regulates HBE1%2C HBG1%2C HBG2%2C HBD%2C HBBP1 and HBD gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280570	5280897	.	.	.	ID=id-GeneID:109580095-35;Dbxref=GeneID:109580095;Note=range from HS2 gRNA 1 to HS2 gRNA 4 that also includes additional chr11.790 gRNAs in the HS2 region targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28369033];function=positively regulates HBE1 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280580	5280590	.	.	.	ID=id-GeneID:109580095-36;Dbxref=GeneID:109580095;Note=HS2 HSC site deleted in the deltaHSC transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8152905];function=weakly contribues to HS2 enhancer activity in transgenic mice;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280628	5280637	.	.	.	ID=id-GeneID:109580095-37;Dbxref=GeneID:109580095;Note=HS2 USF site deleted in the deltaUSF transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8152905];function=required for maximal HS2 enhancer activity in transgenic mice;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5280629	5280650	.	.	.	ID=id-GeneID:109580095-38;Dbxref=GeneID:109580095;Note=8790 E Box fragment that binds the bHLH transcription factor USF;bound_moiety=upstream transcription factor family member;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8360172%2C PMID:8943307];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280631	5280638	.	.	.	ID=id-GeneID:109580095-39;Dbxref=GeneID:109580095;Note=HS2 NF-E footprint;bound_moiety=nuclear factor%2C erythroid 2;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:2000371];function=HS2 enhancer activity;gbkey=protein_bind
NC_000011.10	RefSeqFE	sequence_secondary_structure	5280646	5280660	.	.	.	ID=id-GeneID:109580095-40;Dbxref=GeneID:109580095;Note=region of 5'HS2 that forms a triple helix with the beta-globin (L) tfRNA;experiment=EXISTENCE:electrophoretic mobility shift assay evidence [ECO:0000096][PMID:30867287];function=forms a DNA-RNA triplex with a pyrimidine RNA from intron 2 of the HBB gene%2C resulting in attenuated transcription factor and RNA polymerase II binding to the LCR%2C and reduced expression of the espilon- and gamma-globin genes in transfected K562 cells%2C and the gamma- and beta-globin genes in transfected HUDEP-2 cells {active_cell/tissue: HUDEP-2 | K562};gbkey=misc_structure
NC_000011.10	RefSeqFE	protein_binding_site	5280659	5280672	.	.	.	ID=id-GeneID:109580095-41;Dbxref=GeneID:109580095;Note=8762 E box probe;bound_moiety=TAL bHLH transcription factor 1%2C erythroid differentiation factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8995271];function=binds TAL1 in K562 and MEL cells {active_cell/tissue: K562 | MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280659	5280672	.	.	.	ID=id-GeneID:109580095-42;Dbxref=GeneID:109580095;Note=8762 E box probe;bound_moiety=upstream transcription factor family member;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8995271];function=binds USF in K562 and MEL cells {active_cell/tissue: K562 | MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280693	5280702	.	.	.	ID=id-GeneID:109580095-43;Dbxref=GeneID:109580095;Note=HS2 GATA1 site deleted in the deltaGATA1 transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8152905];function=required for maximal HS2 enhancer activity in transgenic mice;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280695	5280881	.	.	.	ID=id-GeneID:109580095-44;Dbxref=GeneID:109580095;Note=TAD1.SE1.HS2 sgRNA1-sgRNA3 range targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28416141];function=positively regulates HBG1 and HGB2 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5280695	5280709	.	.	.	ID=id-GeneID:109580095-45;Dbxref=GeneID:109580095;Note=inverted GATA-1 binding motifs at HS2;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:chromatin immunoprecipitation evidence [ECO:0000226][PMID:18586828],EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:8995271];function=necessary for activation of the epsilon-globin promoter;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280716	5280745	.	.	.	ID=id-GeneID:109580095-46;Dbxref=GeneID:109580095;Note=8711 E Box fragment that binds the bHLH transcription factor USF%3B weak binding compared to the 8790 E Box fragment;bound_moiety=upstream transcription factor family member;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8943307];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280716	5280745	.	.	.	ID=id-GeneID:109580095-47;Dbxref=GeneID:109580095;Note=HS2NF5 binding fragment%3B HS2 CBF1 binding site;bound_moiety=recombination signal binding protein for immunoglobulin kappa J region;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8943307%2C PMID:9727046];function=important for optimal enhancer activity of HS2;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280719	5280736	.	.	.	ID=id-GeneID:109580095-48;Dbxref=GeneID:109580095;Note=8701 E box probe;bound_moiety=TAL bHLH transcription factor 1%2C erythroid differentiation factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8995271];function=binds TAL1 in K562 and MEL cells {active_cell/tissue: K562 | MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280742	5280747	.	.	.	ID=id-GeneID:109580095-49;Dbxref=GeneID:109580095;Note=HS2 NF-E5 site mutated in the NF-E5S transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8152905];function=weakly contribues to HS2 enhancer activity in transgenic mice;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280752	5280769	.	.	.	ID=id-GeneID:109580095-50;Dbxref=GeneID:109580095;Note=adjacent HS2 AP1/NFE2 sites deleted in the deltaAP1x2 (or NF-E2x2) transgene%2C or mutated in NF-E2 mutant enhancer constructs;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:2235483],EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8152905%2C PMID:2000371];function=required for maximal HS2 enhancer activity in transgenic mice and in induced K562 cells {active_cell/tissue: K562(induced)};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5280753	5280770	.	.	.	ID=id-GeneID:109580095-51;Dbxref=GeneID:109580095;Note=HS2 AP1 site pair bound by NF-E2;bound_moiety=nuclear factor%2C erythroid 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:1902783%2C PMID:8816476%2C PMID:2235483];function=forms HS2 by disruption of nucleosome structure;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280753	5280760	.	.	.	ID=id-GeneID:109580095-52;Dbxref=GeneID:109580095;Note=HS2 AP1 footprint;bound_moiety=Jun proto-oncogene%2C AP-1 transcription factor subunit;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:2000371%2C PMID:8995271];function=HS2 enhancer activity;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5280763	5280770	.	.	.	ID=id-GeneID:109580095-53;Dbxref=GeneID:109580095;Note=HS2 AP1 footprint;bound_moiety=Jun proto-oncogene%2C AP-1 transcription factor subunit;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:2000371%2C PMID:8995271];function=HS2 enhancer activity;gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5280824	5280829	.	.	.	ID=id-GeneID:109580095-54;Dbxref=GeneID:109580095;Note=HS2 SP1 site deleted in the deltaSP1 transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8152905];function=required for maximal HS2 enhancer activity in transgenic mice;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5280824	5280829	.	.	.	ID=id-GeneID:109580095-55;Dbxref=GeneID:109580095;Note=HS2 SP1 footprint;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:nuclease protection assay evidence [ECO:0001106][PMID:2000371];function=HS2 enhancer activity;gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5281118	5281142	.	.	.	ID=id-GeneID:109580095-56;Dbxref=GeneID:109580095;Note=HS2 M1-MAR SATB1 binding site;bound_moiety=SATB homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:15618465];function=enhances transcriptional activity of the epsilon promoter;gbkey=protein_bind
NC_000011.10	RefSeqFE	sequence_secondary_structure	5281205	5281324	.	.	.	ID=id-GeneID:109580095-57;Dbxref=GeneID:109580095;Note=intrinsic bending region%3B epsilonB-17 DNA bend center flanking HS2;experiment=EXISTENCE:gel electrophoresis evidence [ECO:0000337][PMID:9837721];function=nucleosome phasing at HS2;gbkey=misc_structure
NC_000011.10	RefSeqFE	enhancer	5283270	5285590	.	.	.	ID=id-GeneID:109580095-58;Dbxref=GeneID:109580095;Note=2.3 kb SpeI-HindIII HS3 fragment deleted in the beta-YAC LCR transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:8692864];function=enhances epsilon-globin expression in the embryonic yolk sac of transgenic mice {active_cell/tissue: yolk sac(embryonic)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	matrix_attachment_site	5283464	5284059	.	.	.	ID=id-GeneID:109580095-59;Dbxref=GeneID:109580095;Note=596 bp HS3 amplicon showing matrix enrichment in non-erythroid GM00468 cell EcoRI/HindIII-digested DNA fractions;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:12799453];function=attaches to the nuclear matrix in GM00468 cells {active_cell/tissue: GM00468};gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	enhancer	5283753	5286050	.	.	.	ID=id-GeneID:109580095-60;Dbxref=GeneID:109580095;Note=MfeI-HpaI HS3 fragment in the HS3ALL construct;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:14636653];function=enhances beta-globin gene expression in adult bone marrow of transgenic mice {active_cell/tissue: bone marrow(adult)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5283823	5283853	.	.	.	ID=id-GeneID:109580095-61;Dbxref=GeneID:109580095;Note=FP24 oligonucleotide%3B moderate GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283823	5283853	.	.	.	ID=id-GeneID:109580095-62;Dbxref=GeneID:109580095;Note=FP24 oligonucleotide%3B moderate Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283839	5283868	.	.	.	ID=id-GeneID:109580095-63;Dbxref=GeneID:109580095;Note=FP23 oligonucleotide%3B moderate Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283839	5283868	.	.	.	ID=id-GeneID:109580095-64;Dbxref=GeneID:109580095;Note=FP23 oligonucleotide%3B weak GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283854	5283879	.	.	.	ID=id-GeneID:109580095-65;Dbxref=GeneID:109580095;Note=FP22 oligonucleotide%3B moderate Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283869	5283900	.	.	.	ID=id-GeneID:109580095-66;Dbxref=GeneID:109580095;Note=FP21 oligonucleotide%3B strong GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283869	5283900	.	.	.	ID=id-GeneID:109580095-67;Dbxref=GeneID:109580095;Note=FP21 oligonucleotide%3B strong Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283881	5283910	.	.	.	ID=id-GeneID:109580095-68;Dbxref=GeneID:109580095;Note=FP20 oligonucleotide%3B strong GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283881	5283910	.	.	.	ID=id-GeneID:109580095-69;Dbxref=GeneID:109580095;Note=FP20 oligonucleotide%3B weak Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283975	5284013	.	.	.	ID=id-GeneID:109580095-70;Dbxref=GeneID:109580095;Note=FP19 oligonucleotide%3B moderate GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5283975	5284013	.	.	.	ID=id-GeneID:109580095-71;Dbxref=GeneID:109580095;Note=FP19 oligonucleotide%3B weak Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284051	5284083	.	.	.	ID=id-GeneID:109580095-72;Dbxref=GeneID:109580095;Note=FP18 oligonucleotide%3B weak YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284051	5284083	.	.	.	ID=id-GeneID:109580095-73;Dbxref=GeneID:109580095;Note=FP18 oligonucleotide%3B strong USF binding;bound_moiety=upstream transcription factor family member;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds USF in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284079	5284108	.	.	.	ID=id-GeneID:109580095-74;Dbxref=GeneID:109580095;Note=FP17 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284234	5284263	.	.	.	ID=id-GeneID:109580095-75;Dbxref=GeneID:109580095;Note=FP16 oligonucleotide%3B moderate GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds GATA-1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284234	5284263	.	.	.	ID=id-GeneID:109580095-76;Dbxref=GeneID:109580095;Note=FP16 oligonucleotide%3B moderate Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds Oct-1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284234	5284263	.	.	.	ID=id-GeneID:109580095-77;Dbxref=GeneID:109580095;Note=FP16 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5284256	5286162	.	.	.	ID=id-GeneID:109580095-78;Dbxref=GeneID:109580095;Note=1.9 kb HindIII HS3 enhancer fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8662652%2C PMID:10196210];function=chromatin domain-opening activity in stably transfected K562 cells%3B synergizes with the HS2 enhancer to allow stage-specific expression of the gamma- and beta-globin genes in transfected HEL and K562 cells {active_cell/tissue: HEL | K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5284304	5284783	.	.	.	ID=id-GeneID:109580095-79;Dbxref=GeneID:109580095;Note=0.8 kb PvuII-PstI HS3 fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8662652];function=modest enhancer of the epsilon-globin promoter in transfected K562 cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5284328	5284356	.	.	.	ID=id-GeneID:109580095-80;Dbxref=GeneID:109580095;Note=FP15 oligonucleotide%3B weak Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284328	5284356	.	.	.	ID=id-GeneID:109580095-81;Dbxref=GeneID:109580095;Note=FP15 oligonucleotide%3B strong Sp1 binding;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds Sp1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284328	5284356	.	.	.	ID=id-GeneID:109580095-82;Dbxref=GeneID:109580095;Note=FP15 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284446	5284484	.	.	.	ID=id-GeneID:109580095-83;Dbxref=GeneID:109580095;Note=FP14 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5284486	5285080	.	.	.	ID=id-GeneID:109580095-84;Dbxref=GeneID:109580095;Note=PstI-AvaII HS3 fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:12582237];function=enhances transcription of the beta-globin gene in transfected K562 cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5284507	5284536	.	.	.	ID=id-GeneID:109580095-85;Dbxref=GeneID:109580095;Note=FP13 oligonucleotide%3B moderate Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284507	5284536	.	.	.	ID=id-GeneID:109580095-86;Dbxref=GeneID:109580095;Note=FP13 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284507	5284536	.	.	.	ID=id-GeneID:109580095-87;Dbxref=GeneID:109580095;Note=FP13 oligonucleotide%3B weak GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5284514	5285160	.	.	.	ID=id-GeneID:109580095-88;Dbxref=GeneID:109580095;Note=5'HS3%2C also known as HS3a%2C HSS3%2C HSIII or -14.8 hypersensitive site%3B predominantly erythroid%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:2370867%2C PMID:3879975%2C PMID:3480506%2C PMID:3690667];function=necessary for high-level beta-globin gene expression and for position-independent and copy number-dependent activity of the LCR in transgenic mice;gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5284562	5285063	.	.	.	ID=id-GeneID:109580095-89;Dbxref=GeneID:109580095;Note=range from the chr11.791.38 to chr11.791.32 gRNAs in the HS3 region targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28369033];function=positively regulates HBE1 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5284634	5284663	.	.	.	ID=id-GeneID:109580095-90;Dbxref=GeneID:109580095;Note=FP12 oligonucleotide%3B weak GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284634	5284663	.	.	.	ID=id-GeneID:109580095-91;Dbxref=GeneID:109580095;Note=FP12 oligonucleotide%3B moderate NF-E2 binding;bound_moiety=nuclear factor%2C erythroid 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds NF-E2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284634	5284663	.	.	.	ID=id-GeneID:109580095-92;Dbxref=GeneID:109580095;Note=FP12 oligonucleotide%3B moderate Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284634	5284663	.	.	.	ID=id-GeneID:109580095-93;Dbxref=GeneID:109580095;Note=FP12 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds YY1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5284656	5284878	.	.	.	ID=id-GeneID:109580095-94;Dbxref=GeneID:109580095;Note=0.2 kb Fnu4H1-HphI HS3 core (HS3c) fragment deleted in the beta-YAC transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:9632803%2C PMID:16055715];function=necessary for expression of the epsilon-globin gene in embryonic cells and gamma-globin gene in definitive cells of transgenic mice%3B necessary for histone acetylation across the beta-globin locus and for RNA polymerase II recruitment to the LCR;gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5284694	5284894	.	.	.	ID=id-GeneID:109580095-95;Dbxref=GeneID:109580095;Note=TAD1.SE1.HS3 sgRNA1-sgRNA3 range targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28416141];function=positively regulates HBG1 and HGB2 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5284697	5284721	.	.	.	ID=id-GeneID:109580095-96;Dbxref=GeneID:109580095;Note=FP11 oligonucleotide%3B strong GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284697	5284721	.	.	.	ID=id-GeneID:109580095-97;Dbxref=GeneID:109580095;Note=FP11 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284713	5284742	.	.	.	ID=id-GeneID:109580095-98;Dbxref=GeneID:109580095;Note=FP10 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds YY1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284725	5284755	.	.	.	ID=id-GeneID:109580095-99;Dbxref=GeneID:109580095;Note=FP9 oligonucleotide%3B strong Sp1 binding;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds Sp1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284725	5284755	.	.	.	ID=id-GeneID:109580095-100;Dbxref=GeneID:109580095;Note=FP9 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284749	5284782	.	.	.	ID=id-GeneID:109580095-101;Dbxref=GeneID:109580095;Note=FP8 oligonucleotide%3B weak YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284749	5284782	.	.	.	ID=id-GeneID:109580095-102;Dbxref=GeneID:109580095;Note=FP8 oligonucleotide%3B weak GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284781	5284820	.	.	.	ID=id-GeneID:109580095-103;Dbxref=GeneID:109580095;Note=footprint 3 (FP3) or FP7 oligonucleotide%3B strong GATA-1-binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325%2C PMID:9129054];function=binds GATA-1 in K562 and MEL erythroleukemia cells {active_cell/tissue: K562 | MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284793	5284823	.	.	.	ID=id-GeneID:109580095-104;Dbxref=GeneID:109580095;Note=FP7 oligonucleotide%3B weak YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284814	5284852	.	.	.	ID=id-GeneID:109580095-105;Dbxref=GeneID:109580095;Note=footprint 2 (FP2) Sp1-binding oligonucleotide;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds Sp1 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284814	5284852	.	.	.	ID=id-GeneID:109580095-106;Dbxref=GeneID:109580095;Note=footprint 3 (FP3) TEF-2-binding oligonucleotide;bound_moiety=speckle type BTB/POZ protein;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325];function=binds TEF-2 in murine erythroleukemia cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284843	5284881	.	.	.	ID=id-GeneID:109580095-107;Dbxref=GeneID:109580095;Note=FP6 oligonucleotide%3B weak YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284845	5284878	.	.	.	ID=id-GeneID:109580095-108;Dbxref=GeneID:109580095;Note=footprint 1 (FP1) or FP6 oligonucleotide%3B moderate GATA-1-binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:8458325%2C PMID:9129054];function=binds GATA-1 in K562 and MEL erythroleukemia cells {active_cell/tissue: K562 | MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284899	5284928	.	.	.	ID=id-GeneID:109580095-109;Dbxref=GeneID:109580095;Note=FP4 oligonucleotide%3B weak Oct-1 binding;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds Oct-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284899	5284928	.	.	.	ID=id-GeneID:109580095-110;Dbxref=GeneID:109580095;Note=FP4 oligonucleotide%3B moderate NF-E2 binding;bound_moiety=nuclear factor%2C erythroid 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds NF-E2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284899	5284928	.	.	.	ID=id-GeneID:109580095-111;Dbxref=GeneID:109580095;Note=FP4 oligonucleotide%3B weak GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284978	5285007	.	.	.	ID=id-GeneID:109580095-112;Dbxref=GeneID:109580095;Note=FP3 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5284978	5285007	.	.	.	ID=id-GeneID:109580095-113;Dbxref=GeneID:109580095;Note=FP3 oligonucleotide%3B weak GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5285157	5285186	.	.	.	ID=id-GeneID:109580095-114;Dbxref=GeneID:109580095;Note=FP1 oligonucleotide%3B strong GATA-1 binding;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds GATA-1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5285157	5285186	.	.	.	ID=id-GeneID:109580095-115;Dbxref=GeneID:109580095;Note=FP1 oligonucleotide%3B strong YY1 binding;bound_moiety=YY1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9129054];function=binds YY1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5285401	5286115	.	.	.	ID=id-GeneID:109580095-116;Dbxref=GeneID:109580095;Note=5'HS3.5%2C also known as HS3.5%2C HS3b%2C HS3.2 or secondary HS3%3B weak and predominantly erythroid%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:3690667%2C PMID:7828582];function=domain-opening activity;gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	enhancer	5285990	5288872	.	.	.	ID=id-GeneID:109580095-117;Dbxref=GeneID:109580095;Note=2.9 kb StuI fragment deleted in the delta-5'HS4 beta-YAC transgene;experiment=EXISTENCE:deletion mutation evidence [ECO:0005510][PMID:17433733];function=weakly enhances beta-globin gene expression in day 12 fetal liver in transgenic mice {active_cell/tissue: liver(fetal)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5286081	5286110	.	.	.	ID=id-GeneID:109580095-118;Dbxref=GeneID:109580095;Note=HS3.2 6005 GATA1- and Oct1-binding oligonucleotide;bound_moiety=POU class 2 homeobox 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:11867225];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5286081	5286110	.	.	.	ID=id-GeneID:109580095-119;Dbxref=GeneID:109580095;Note=HS3.2 6005 GATA1- and Oct1-binding oligonucleotide;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:11867225];gbkey=protein_bind
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5287851	5288585	.	.	.	ID=id-GeneID:109580095-120;Dbxref=GeneID:109580095;Note=5'HS4%2C also known as HS4%2C HSS4%2C HSIV%2C -18 or -18.4 hypersensitive site%3B predominantly erythroid%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:2370867%2C PMID:3879975%2C PMID:3480506%2C PMID:3690667%2C PMID:7828582%2C PMID:1736298];function=necessary for high-level beta-globin gene expression and for position-independent activity of the LCR in transgenic mice;gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	enhancer	5288090	5288474	.	.	.	ID=id-GeneID:109580095-121;Dbxref=GeneID:109580095;Note=SacI-SspI HS4 fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:12582237];function=enhances transcription of the beta-globin gene in transfected K562 cells%3B EKLF-dependent activity {active_cell/tissue: K562(+EKLF)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5288097	5288387	.	.	.	ID=id-GeneID:109580095-122;Dbxref=GeneID:109580095;Note=range from the chr11.792.5 to chr11.792.3 gRNAs in the HS4 region targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28369033];function=positively regulates HBE1 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5288183	5288348	.	.	.	ID=id-GeneID:109580095-123;Dbxref=GeneID:109580095;Note=TAD1.SE1.HS4 sgRNA1-sgRNA3 range targeted for Mosaic-seq CRISPR perturbation;experiment=EXISTENCE:mutant phenotype evidence [ECO:0000015][PMID:28416141];function=positively regulates HBG1 and HGB2 gene expression in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5288193	5288205	.	.	.	ID=id-GeneID:109580095-124;Dbxref=GeneID:109580095;Note=HS4 Sp1-binding site%3B oligo 920 fragment;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288193	5288205	.	.	.	ID=id-GeneID:109580095-125;Dbxref=GeneID:109580095;Note=HS4 CACC- or TEF-2-binding site%3B oligo 920 fragment;bound_moiety=speckle type BTB/POZ protein;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748];gbkey=protein_bind
NC_000011.10	RefSeqFE	enhancer	5288194	5288473	.	.	.	ID=id-GeneID:109580095-126;Dbxref=GeneID:109580095;Note=280 bp SacI-AvaI fragment deleted in the deltaHS4c beta-YAC transgene;experiment=EXISTENCE:deletion mutation evidence [ECO:0005510][PMID:2027748%2C PMID:11545582];function=enhances gamma- and beta-globin gene expression during definitive erythropoiesis in fetal liver and beta-globin expression in adult blood in transgenic mice {active_cell/tissue: blood(adult) | liver(fetal)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	protein_binding_site	5288210	5288232	.	.	.	ID=id-GeneID:109580095-127;Dbxref=GeneID:109580095;Note=HS4 CACC- or TEF-2-binding site%3B oligo 890 fragment;bound_moiety=speckle type BTB/POZ protein;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288210	5288232	.	.	.	ID=id-GeneID:109580095-128;Dbxref=GeneID:109580095;Note=HS4 Sp1 binding site%3B oligo 890 fragment;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748];gbkey=protein_bind
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5288230	5288331	.	.	.	ID=id-GeneID:109580095-129;Dbxref=GeneID:109580095;Note=101 bp HS4 HS-forming element (HSFE);experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:7828582];function=required for HS4 formation in stably transfected MEL cells {active_cell/tissue: MEL};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	protein_binding_site	5288240	5288264	.	.	.	ID=id-GeneID:109580095-130;Dbxref=GeneID:109580095;Note=HS4 HS-forming element inverted GATA-1 sites%3B oligo 870 fragment;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748%2C PMID:7828582];function=required for HS4 formation in stably transfected MEL cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288269	5288288	.	.	.	ID=id-GeneID:109580095-131;Dbxref=GeneID:109580095;Note=HS4 HS-forming element SP-1 site%3B oligo 840 fragment;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748%2C PMID:7828582];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288269	5288288	.	.	.	ID=id-GeneID:109580095-132;Dbxref=GeneID:109580095;Note=HS4 CACC- or TEF-2-binding site%3B oligo 840 fragment;bound_moiety=speckle type BTB/POZ protein;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288294	5288311	.	.	.	ID=id-GeneID:109580095-133;Dbxref=GeneID:109580095;Note=HS4 HS-forming element AP-1/NF-E2 site%3B oligo 810 fragment;bound_moiety=Jun proto-oncogene%2C AP-1 transcription factor subunit;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748%2C PMID:7828582];function=required for HS4 formation in stably transfected MEL cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288294	5288311	.	.	.	ID=id-GeneID:109580095-134;Dbxref=GeneID:109580095;Note=HS4 HS-forming element AP-1/NF-E2 site%3B oligo 810 fragment;bound_moiety=nuclear factor%2C erythroid 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:2027748%2C PMID:7828582];function=required for HS4 formation in stably transfected MEL cells {active_cell/tissue: MEL};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288338	5288367	.	.	.	ID=id-GeneID:109580095-135;Dbxref=GeneID:109580095;Note=HS4 CBF1 binding site;bound_moiety=recombination signal binding protein for immunoglobulin kappa J region;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:9727046];gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5288450	5288478	.	.	.	ID=id-GeneID:109580095-136;Dbxref=GeneID:109580095;Note=5'HS4 RUNX1 allele-specific palindromic binding site;bound_moiety=runt related transcription factor 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:27492765];gbkey=protein_bind
NC_000011.10	RefSeqFE	insulator	5289115	5292124	.	.	.	ID=id-GeneID:109580095-137;Dbxref=GeneID:109580095;Note=3 kb 5'HS5 fragment in the micro-gamma-5 transgene%3B HindIII-BamHI betaGlb-MAR or beta-MAR fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:8112598],EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:12034837];function=protects an Agamma promoter transgene from position effects in transgenic mice but only when it brackets the transgene%3B insulates against position effects and confers high levels of copy number-dependent transcription on a polyoma enhancer in stably transfected HT1080 cells {active_cell/tissue: HT1080};gbkey=regulatory;regulatory_class=insulator
NC_000011.10	RefSeqFE	insulator	5289327	5290163	.	.	.	ID=id-GeneID:109580095-138;Dbxref=GeneID:109580095;Note=5'HS5A fragment in micro-gamma-5A transgene;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:12034837];function=protects a position-sensitive Agamma promoter transgene from position effects in embryonic but not adult stage transgenic mice%3B not copy number-dependent;gbkey=regulatory;regulatory_class=insulator
NC_000011.10	RefSeqFE	matrix_attachment_site	5289426	5292315	.	.	.	ID=id-GeneID:109580095-139;Dbxref=GeneID:109580095;Note=EcoRI-BglII pGSE271 fragment b SAR encompassing 5'HS5;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:3208739%2C PMID:2559410];function=associates with the nuclear scaffold;gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	enhancer_blocking_element	5289471	5292124	.	.	.	ID=id-GeneID:109580095-140;Dbxref=GeneID:109580095;Note=2.6 kb HS5 HindIII fragment;experiment=EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:14645507];function=developmental%2C stage-specific enhancer-blocking activity in transgenic mice;gbkey=regulatory;regulatory_class=enhancer_blocking_element
NC_000011.10	RefSeqFE	matrix_attachment_site	5290091	5290490	.	.	.	ID=id-GeneID:109580095-141;Dbxref=GeneID:109580095;Note=393 bp HS5 amplicon showing matrix enrichment in K562 cell EcoRI/HindIII- and EcoRI/BamHI-digested DNA fractions;experiment=EXISTENCE:fractionation evidence [ECO:0000100][PMID:12799453];gbkey=regulatory;regulatory_class=matrix_attachment_region
NC_000011.10	RefSeqFE	silencer	5290100	5290139	.	.	.	ID=id-GeneID:109580095-142;Dbxref=GeneID:109580095;Note=(GATA)7 5a sequence;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:10936858];function=represses HS2 enhancer activity in transfected K562 and HEL cells {active_cell/tissue: HEL | K562};gbkey=regulatory;regulatory_class=silencer
NC_000011.10	RefSeqFE	protein_binding_site	5290100	5290139	.	.	.	ID=id-GeneID:109580095-143;Dbxref=GeneID:109580095;Note=(GATA)7 5a sequence;bound_moiety=GATA binding protein 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:10936858];function=represses HS2 enhancer activity;gbkey=protein_bind
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5290804	5291803	.	.	.	ID=id-GeneID:109580095-144;Dbxref=GeneID:109580095;Note=5'HS5%2C also known as HS5%2C HSS5%2C HSV or -21.4 hypersensitive site%3B not exclusively erythroid%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:2370867%2C PMID:3879975%2C PMID:12941700];function=enhancer-blocking activity;gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeqFE	transcriptional_cis_regulatory_region	5290878	5292124	.	.	.	ID=id-GeneID:109580095-145;Dbxref=GeneID:109580095;Note=1.2 kb HS5 fragment in the HS5-epsilon-p-CAT construct;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:9878258];function=synergizes with the ERV-9 LTR enhancer in stably transfected K562 cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=transcriptional_cis_regulatory_region
NC_000011.10	RefSeqFE	enhancer_blocking_element	5291404	5291475	.	.	.	ID=id-GeneID:109580095-146;Dbxref=GeneID:109580095;Note=h5'HS5 enhancer-blocking fragment;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:11997516];function=blocks activation of the Agamma-globin promoter by the mouse 5'HS2 enhancer in transfected K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=enhancer_blocking_element
NC_000011.10	RefSeqFE	protein_binding_site	5291404	5291475	.	.	.	ID=id-GeneID:109580095-147;Dbxref=GeneID:109580095;Note=5'HS5 CTCF-binding oligonucleotide;bound_moiety=CCCTC-binding factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:11997516%2C PMID:12861010%2C PMID:18461170];function=binds CTCF and is necessary for enhancer-blocking activity {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	Curated Genomic	pseudogene	5291467	5292351	.	-	.	ID=gene-OR51AB1P;Dbxref=GeneID:403265,HGNC:HGNC:31278;Name=OR51AB1P;description=olfactory receptor family 51 subfamily AB member 1 pseudogene;gbkey=Gene;gene=OR51AB1P;gene_biotype=pseudogene;pseudo=true
NC_000011.10	RefSeqFE	mobile_genetic_element	5293260	5294954	.	.	.	ID=id-GeneID:109580095-148;Dbxref=GeneID:109580095;Note=ERV-9 erythroid enhancer upstream of 5'HS5;gbkey=mobile_element;mobile_element_type=retrotransposon:LTR12C;rpt_family=LTR/ERV1;rpt_type=long_terminal_repeat
NC_000011.10	RefSeqFE	enhancer	5293260	5294363	.	.	.	ID=id-GeneID:109580095-149;Dbxref=GeneID:109580095;Note=1.1 kb fragment of the LTR-GFP construct spanning 14 U3 enhancer repeats%2C the U3 promoter%2C and the R and U5 regions of the ERV-9 LTR;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:9878258%2C PMID:11836419],EXISTENCE:transgenic organism evidence [ECO:0001131][PMID:14718667];function=transcriptional enhancer in transfected K562%2C embryonic and hematopoietic cells%2C and in oocytes and progenitor cells of transgenic zebrafish%3B activates transcription of downstream DNA preferentially in erythroid cells {active_cell/tissue: hematopoietic | K562 | oocytes(transgenic zebrafish) | progenitor cells(transgenic zebrafish)};gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	enhancer	5293620	5294964	.	.	.	ID=id-GeneID:109580095-150;Dbxref=GeneID:109580095;Note=(E-P-r)-GFP construct fragment containing the U3 enhancer and promoter and 5' half of the R region;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:11836419];function=ERV-9 LTR enhancer activity in various embryonic tissues and in adult blood lineage cells;gbkey=regulatory;regulatory_class=enhancer
NC_000011.10	RefSeqFE	promoter	5293675	5293762	.	.	.	ID=id-GeneID:109580095-151;Dbxref=GeneID:109580095;Note=U3 promoter region in the ERV-9 LTR;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:11836419];function=transcription of the R region and downstream sequence;gbkey=regulatory;regulatory_class=promoter
NC_000011.10	RefSeqFE	TATA_box	5293700	5293705	.	.	.	ID=id-GeneID:109580095-152;Dbxref=GeneID:109580095;Note=AATAAA motif 25 bases upstream of the transcription initiation site;experiment=EXISTENCE:motif similarity evidence [ECO:0000028][PMID:9878258%2C PMID:11836419];function=TATA box for the U3 promoter in the ERV-9 LTR;gbkey=regulatory;regulatory_class=TATA_box
NC_000011.10	RefSeqFE	protein_binding_site	5293703	5293763	.	.	.	ID=id-GeneID:109580095-153;Dbxref=GeneID:109580095;Note=NF-Y-binding promoter probe P;bound_moiety=nuclear transcription factor Y subunit;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds NF-Y in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5293703	5293763	.	.	.	ID=id-GeneID:109580095-154;Dbxref=GeneID:109580095;Note=GATA-2-binding promoter probe P;bound_moiety=GATA binding protein 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds GATA-2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5293703	5293763	.	.	.	ID=id-GeneID:109580095-155;Dbxref=GeneID:109580095;Note=Sp1-binding promoter probe P;bound_moiety=Sp1 transcription factor;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds Sp1 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5293926	5293977	.	.	.	ID=id-GeneID:109580095-156;Dbxref=GeneID:109580095;Note=E2 GATA-2-binding probe;bound_moiety=GATA binding protein 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds GATA-2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294015	5294051	.	.	.	ID=id-GeneID:109580095-157;Dbxref=GeneID:109580095;Note=E2 NF-Y-binding probe%3B binds to CCAAT motif;bound_moiety=nuclear transcription factor Y subunit;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds NF-Y in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294015	5294051	.	.	.	ID=id-GeneID:109580095-158;Dbxref=GeneID:109580095;Note=E4 GATA-2-binding probe;bound_moiety=GATA binding protein 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds GATA-2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294016	5294041	.	.	.	ID=id-GeneID:109580095-159;Dbxref=GeneID:109580095;Note=E4 (MZF) MZF1-binding probe;bound_moiety=myeloid zinc finger 1;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds MZF in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294086	5294137	.	.	.	ID=id-GeneID:109580095-160;Dbxref=GeneID:109580095;Note=E2 NF-Y-binding probe%3B binds to CCAAT motif;bound_moiety=nuclear transcription factor Y subunit;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds NF-Y in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294086	5294137	.	.	.	ID=id-GeneID:109580095-161;Dbxref=GeneID:109580095;Note=E2 GATA-2-binding probe;bound_moiety=GATA binding protein 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds GATA-2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294134	5294174	.	.	.	ID=id-GeneID:109580095-162;Dbxref=GeneID:109580095;Note=E1 GATA-2-binding probe;bound_moiety=GATA binding protein 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds GATA-2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294134	5294174	.	.	.	ID=id-GeneID:109580095-163;Dbxref=GeneID:109580095;Note=E1 NF-Y-binding probe%3B binds to CCAAT motif;bound_moiety=nuclear transcription factor Y subunit;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds NF-Y in K562 erythroleukemia and PA-1 teratocarcinoma cells {active_cell/tissue: K562 | PA-1};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294244	5294295	.	.	.	ID=id-GeneID:109580095-164;Dbxref=GeneID:109580095;Note=E2 NF-Y-binding probe%3B binds to CCAAT motif;bound_moiety=nuclear transcription factor Y subunit;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds NF-Y in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	protein_binding_site	5294244	5294295	.	.	.	ID=id-GeneID:109580095-165;Dbxref=GeneID:109580095;Note=E2 GATA-2-binding probe;bound_moiety=GATA binding protein 2;experiment=EXISTENCE:protein binding evidence [ECO:0000024][PMID:16105833];function=binds GATA-2 in K562 erythroleukemia cells {active_cell/tissue: K562};gbkey=protein_bind
NC_000011.10	RefSeqFE	DNaseI_hypersensitive_site	5297387	5298186	.	.	.	ID=id-GeneID:109580095-166;Dbxref=GeneID:109580095;Note=5'HS6%2C also known as HS6 or HSVI%3B observed in fetal liver but not fetal brain%3B the nucleotide coordinates are approximate for this feature;experiment=EXISTENCE:in vivo cleavage assay evidence [ECO:0001075][PMID:10220430];function=chromatin accessibility in fetal liver {active_cell/tissue: liver(fetal)};gbkey=regulatory;regulatory_class=DNase_I_hypersensitive_site
NC_000011.10	RefSeq	match	5215910	5297615	81706	+	.	ID=7f9751c7-2d4d-4aa7-b763-be9bd2e8aeb4;Target=NG_000007.3 1 81706 -;assembly_bases_aln=81706;assembly_bases_seq=81706;bit_score=152062;blast_aligner=1;blast_score=81706;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=81706;matched_bases=81706;not_for_annotation=2;num_ident=81706;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5221966	5232071	10106	+	.	ID=4ae3b117-6625-41ea-9dc3-7e899c0feea7;Target=NG_059281.1 1 10106 -;assembly_bases_aln=10106;assembly_bases_seq=10106;bit_score=18809.1;blast_aligner=1;blast_score=10106;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=10106;matched_bases=10106;not_for_annotation=2;num_ident=10106;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5222066	5229720	7655	+	.	ID=212ebfd9-21ca-40d5-9b29-cc4753f73614;Target=NG_046672.1 1 7655 +;assembly_bases_aln=7655;assembly_bases_seq=7655;bit_score=14247.6;blast_aligner=1;blast_score=7655;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=7655;matched_bases=7655;num_ident=7655;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5223219	5223627	409	+	.	ID=a85aa73e-c385-4025-94a5-3d230566d378;Target=NG_159868.1 1 409 +;assembly_bases_aln=409;assembly_bases_seq=409;bit_score=762.209;blast_aligner=1;blast_score=409;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=409;matched_bases=409;num_ident=409;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5223680	5226690	3011	+	.	ID=81f07ba3-6a0b-49e5-93cc-717b75521b72;Target=NG_053049.1 1 3011 +;assembly_bases_aln=3011;assembly_bases_seq=3011;bit_score=5604.73;blast_aligner=1;blast_score=3011;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=3011;matched_bases=3011;num_ident=3011;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5226470	5228934	2465	+	.	ID=c3d8adce-3373-4a5a-aa17-42cd6522fc46;Target=NG_042296.1 1 2465 +;assembly_bases_aln=2465;assembly_bases_seq=2465;bit_score=4588.58;blast_aligner=1;blast_score=2465;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=2465;matched_bases=2465;num_ident=2465;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5230678	5248657	17980	+	.	ID=acdafb2d-deae-41e0-870e-ec58ab7dda32;Target=NG_063112.2 1 17980 -;assembly_bases_aln=17980;assembly_bases_seq=17980;bit_score=33463.2;blast_aligner=1;blast_score=17980;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=17980;matched_bases=17980;not_for_annotation=2;num_ident=17980;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5233884	5234465	582	+	.	ID=b91e6d73-4f0a-40d9-b3e6-78de7d7a0020;Target=NG_042297.1 1 582 +;assembly_bases_aln=582;assembly_bases_seq=582;bit_score=1084.18;blast_aligner=1;blast_score=582;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=582;matched_bases=582;num_ident=582;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5234333	5239483	5151	+	.	ID=e444ddd6-504f-4b40-9b9a-733604fc2a28;Target=NG_053024.1 1 5151 +;assembly_bases_aln=5151;assembly_bases_seq=5151;bit_score=9587.43;blast_aligner=1;blast_score=5151;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=5151;matched_bases=5151;num_ident=5151;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5245427	5248445	3019	+	.	ID=e615c0ed-9a44-4daf-93c8-72b2404518da;Target=NG_053023.1 1 3019 +;assembly_bases_aln=3019;assembly_bases_seq=3019;bit_score=5619.62;blast_aligner=1;blast_score=3019;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=3019;matched_bases=3019;num_ident=3019;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5248968	5250424	1457	+	.	ID=715723c3-d419-48e3-8405-b28876d36eda;Target=NG_042298.1 1 1457 +;assembly_bases_aln=1457;assembly_bases_seq=1457;bit_score=2712.62;blast_aligner=1;blast_score=1457;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=1457;matched_bases=1457;num_ident=1457;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5253887	5255352	1466	+	.	ID=278841d4-d6fb-4e29-93fa-700ce81ecb03;Target=NG_042299.1 1 1466 +;assembly_bases_aln=1466;assembly_bases_seq=1466;bit_score=2729.37;blast_aligner=1;blast_score=1466;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=1466;matched_bases=1466;num_ident=1466;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5257161	5257976	816	+	.	ID=bb0da93d-a8e7-4c2a-8b62-cdf59cdacef8;Target=NG_121741.1 1 816 +;assembly_bases_aln=816;assembly_bases_seq=816;bit_score=1519.67;blast_aligner=1;blast_score=816;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=816;matched_bases=816;num_ident=816;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5266673	5267619	947	+	.	ID=4d38edc9-b008-4a87-8c90-82f76be8dc9b;Target=NG_055459.1 1 947 +;assembly_bases_aln=947;assembly_bases_seq=947;bit_score=1763.47;blast_aligner=1;blast_score=947;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=947;matched_bases=947;num_ident=947;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5269825	5304286	34462	+	.	ID=359a837e-e451-49b7-8934-9ac512f47258;Target=NG_052895.1 1 34462 +;assembly_bases_aln=34462;assembly_bases_seq=34462;bit_score=64137.5;blast_aligner=1;blast_score=34462;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=34462;matched_bases=34462;num_ident=34462;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1
NC_000011.10	RefSeq	match	5291367	5292451	1085	+	.	ID=fdf009d5-a60f-49c2-8882-22dee871c057;Target=NG_004424.1 1 1085 -;assembly_bases_aln=1085;assembly_bases_seq=1085;bit_score=2020.3;blast_aligner=1;blast_score=1085;common_component=0;e_value=0;filter_score=0;for_remapping=2;gap_count=0;hsp_percent_coverage=100;matchable_bases=1085;matched_bases=1085;num_ident=1085;num_mismatch=0;pct_coverage=100;pct_coverage_hiqual=100;pct_identity_gap=100;pct_identity_gapopen_only=100;pct_identity_ungap=100;rank=1;weighted_identity=1